Signagen/GenJet Plus DNA In Vitro Transfection Reagent/SL100499/5 x 1.0 mL,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/GenJet Plus DNA In Vitro Transfection Reagent/SL100499/5 x 1.0 mL

产品编号：SL100499-5x1.0mL

市场价：¥17560.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$878.00

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

GenJetPlusDNAInVitroTransfectionReagent

Description
GenJetPlusDNAInVitroTransfectionReagentisenhancedversionofGenJetDNAInVitroTransfectionReagent.GenJetPlusisformulatedbyadditionofaboosterpeptide,givingrisetoupto20timeshigherefficiencyonvarietyofmammaliancellsincomparisonofitspreviousversion.GenJetPluswasshowntoefficientlydelivergenestovariousestablishedcelllinesaswellasprimarycellsincludingHEK293,293T,293E,CHO,COS1,HeLa,NIH3T3,insectcelllines(Sf9andSf21)andavarietyofothereucaryoticcelllineswithlesstoxicity.GenJetPlusreagent,1.0ml,issufficientfor667transfectionsin24wellplatesor333transfectionsin6wellplates.

FeaturescomparedwitholdversionGenJetInVitroDNATransfectionReagent
-Cell-dependent3~20timeshigherefficiency
-Topchoiceforhard-to-transfectcells
-EquallygoodforverylongDNAs(upto46kb)
-Efficiencyboostedinthepresenceofserumandantibioticsformostofcelltypes
-Exceptionalhighlevelsofrecombinantproteinproduction

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.

ComparisonsofTransfectionEfficiencyofGenJetPlusInVitroDNATransfectionReagentwiththeLeADIngBrandNameProducts

GenJetPlusistopchoiceforhard-to-transfectcells.EfficiencycomparisonofGenJetPlusInVitroDNATransfectionReagent(leftpanel)withLipofectamine2000(L2K,rightpanel)onprimaryculturedrataorticsmoothmusclecells.AnucleartargetedGFPDNAwastransfectedwithGenJetPlusandL2Krespectivelypermanufacturer"sprotocols.Theefficiencywaschecked48hoursposttransfection.TheabovepictureswerekindlyprovidedbyDr.NickolaiDulinofSectionofPulmonaryandCriticalCare,UniversityofChicago

LeftPanel:ComparisonofGenJetPlusInVitroDNATransfectionReagentwithleadingproductsincludingLipofectamine2000(L2K)andLipofectamineLTX(LTX)onlentivirusproductionon293Tcells.ThedatawereprovidedbyDr.MiguelToscanoofTempleUniversityascourtesy.
Rightpanel:ComparisonofGenJetPlusInVitroDNATransfectionReagentwithtransfectionreagentsfromothervendorsinludingLipofectamine2000(L2K)andFuGene6onHelacells.HeLacellsweretransfectedwithplasmidDNAencodingGFP.Transfectionswereperformedaccordingtomanufacturersrecommendationsandanalysedbyflowcytometry.

Exceptionalhightitersoflentivirusgeneratedfrom293Tcells.ThreeDNAsco-transfectedwithGenJetPlusInVitroDNATransfectionReagent(leftpanel)incomparisonofLipofectamineLTX(rightpanel).1.0and10microlitersvirussupernatantfrom293Tcellswereusedtocounter-infected293FcellswhichwasthenpassedFACS.Thenumbersattheupperrightcornerindicatethepercentageoftransducedcells.ThetitersoflentivirusgeneratedwithGenJetPlusandLipofectamineLTXwerequantifiedtobe2.1x10⁶and9.3x10⁶tu/mlrespectively.TheabovedatawereprovidedbyDr.MiguelToscanoofTempleUniversityascourtesy.

TechnicalInformation&Datasheet
-GenJetPlusDataSheet&GeneralProtocol
-GenJetPlusInVitroDNATransfectionforLentivirusProduction
-GenJetPlusInVitroDNATransfectionforrAAVProduction
-TransfectionofSf9InsectCellswithGenJet

RelatedProduct:GenJetInVitroDNATransfectionreagent

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。