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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/GenJet™ In Vitro DNA Transfection Reagent/SL100488/1.0 mL
产品编号:SL100488-5x1.0mL
市  场 价:¥14820.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$741.00
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/GenJet™ In Vitro DNA Transfection Reagent/SL100488/1.0 mL
商品介绍
GenJet™InVitroDNATransfectionReagent

Description

GenJet™DNAInVitroTranfectionReagentisapowerfultransfectionreagentthatensureseffectiveandreproducIBLetransfectionwithinvisibletoxicity.GenJet™isformulatedbyuniquechemistry(covalentlycross-linkingcationiclipidswithpolymer),givingrisetoexceptionaltransfectionefficiencywithdistinguishablefeaturesincomparisonofothertypesofreagents.GenJet™wasshowntodelivergenestovariousestablishedcelllinesaswellasprimarycellsincludingHEK293,293T,293E,CHO,COS1,HeLa,NIH3T3,insectcelllines(Sf9andSf21)andavarietyofothereucaryoticcelllines.GenJet™reagent,1.0ml,issufficientfor~666transfectionsin24wellplatesor~333transfectionsin6wellplates.

Application
-DeliverDNA/RNAintomammalian,insectcells
-Greatlyenhance(upto200timeshigherefficiency)virus(e.g.,replication-deficientAdenovirus)infectionofhard-to-transfectcells

Features
-Exceptionalhightitersofvirusproduction
-EquallygoodforverylongDNAs(upto56kb)
-EquallygoodforsingleormultipleDNAtransfections
-Exceptionalhighlevelsofrecombinantproteinproduction
-Topchoiceforhard-to-transfectcells
-Inexpensivetransfectionreagent

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.

BroadTransfectionSpectrumforMammalianCellTypes

CellLines

TransfectionEfficiency(%GFP)

CellLines

TransfectionEfficiency(%GFP)

3LL
B16-F10
BAEC
BHK-21
CaSki
CaCo-2
CHO
HCS-2/8
HEK-293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20
SK-N-SH
McArdle7777
Hep3D
SHEP
MDA-MB-231
3T3-442A
COS-7
CV-1
D407
DHDPro.b
LS180

51%
72%
52%
86%
78%
32%
83%
42%
80-90%
81%
50-65%
49%
22%
38%
20%
60%
78%
71%
38%
10%
60-70%
53%
62%
51%
46%

K562
L929
MCF-7
MDCK
Neuro2A
NIH3T3
PC12
SH-SY5Y
SiHa
SKOV3
HUVEC
IGROV1
Jurkat
6CSFMEo
WEHI231
SAOS-2
SN56
MC3T3-E1
BT474
C2C12
Primarymelanocyte
Hela-S3
A549
HepG2

22%
52%
51%
52%
72%
88%
34%
14%
62%
52%
51%
21%
5%-15%
73%
25%
52%
69%
70%
46%
46%
46%
78%
78%
58%


ComparisonsofTransfectionEfficiencyandCytotoxicityofGenJet™DNAInVitroTransfectionReagentwiththeLeADIngBrandNameProducts

ExceptionaltransfectionefficiencyofextremelylongDNAbyGenJet™DNAInVitroTransfectionReagent.HEK293cellsweretransfectedwithGFPtaggedDNA(total14kb)byGenJet™(upperpanel)andFuGene™6(lowerpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphase(leftpanel)andFITC(rightpanel)imaging24hoursposttransfection

Exceptionalhightitersoflentivirusgeneratedfrom293Fcells.ThreeDNAsco-transfectedwithGenJet™InVitroDNATransfectionReagent(leftpanel)incomparisonofLipoFectamine2000™(L2K,rightpanel).100microlitervirussupernatantfrom293Fcellswereusedtocounter-infected293FcellswhichwasthenpassedFACS.Thenumbersattheupperrightcornerindicatethepercentageoftransducedcells.ThetitersoflentivirusgeneratedwithGenJet™andL2Kwerequantifiedtobe7.9x106and3.1x106tu/mlrespectively

PrimaryMurineSkinFibroblasttransfectedwithpEGFP-C1plasmidusingGenJet™InVitroDNATransfectionReagent(upperpanel)andthemostpopularbrandproductFuGene™HD(lowerpanel).ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging(leftpanel)andFITCimaging(rightpanel)24hourspost-transfection

PrimaryMurineKeratinocytestransfectedwithpEGFP-C1plasmidusingGenJet™InVitroDNATransfectionReagent(upperpanel)andoneofthemostpopularbrandproductFuGene™HD(lowerpanel).ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging(leftpanel)andFITCimaging(rightpanel)24hourspost-transfection

ComparisonofcytotoxicityofGenJet™DNAInVitroTransfectionReagentwithL2K™onprimarymurineskinfibroblast.Theprimarymurinefibroblastwasincubatedwiththeindicatedtransfectionreagents/pEGFP-C1(DNA)complexesabovefor4hoursinserum-freeDMEMHighGlucosemediumfollowedbyreplacementofcompleteserum-containingmedium.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging24hoursposttransfection


TechnicalInformation&Datasheet

-GenJet™InVitroDNATransfectionDatasheet
-GenJet™MSDS


 

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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