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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/GenJet™ In Vitro DNA Transfection Reagent (Ver. II)/SL100489/5 x 1.0 mL
产品编号:SL100489-5x1.0mL
市  场 价:¥15760.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$788.00
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/GenJet™ In Vitro DNA Transfection Reagent (Ver. II)/SL100489/5 x 1.0 mL
商品介绍
GenJet™InVitroDNATransfectionReagent(VersionII)

Description
GenJet™InVitroDNATransfectionReagent(Ver.II)isupgradedversionofGenJet™InVitroDNATransfectionReagent.Adoptinganewchemistry,moreDNAcondensinggroupswereliberatedinGenJet™(Ver.II)(100%vs.80%inoldversion),leADIngto3~4foldsincreaseoftransfectionefficiencywithoutsacrificingcellviABIlity.GenJet™(Ver.II)reagent,1.0ml,issufficientfor~667transfectionsin24wellplatesor~333transfectionsin6wellplates.

FeaturescomparedwitholdversionGenJet™InVitroDNATrasnfectionReagent
-Cell-dependent3~4timeshigherefficiency
-Topchoiceforhard-to-transfectcells
-Efficiencyboostedinthepresenceofserumandantibioticsformostofcelltypes
-Exceptionalhighlevelsofrecombinantproteinproduction

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.

ComparisonsofTransfectionEfficiencyandCytotoxicityofGenJet™(Ver.II)withOldVersionGenJet™andOtherLeadingProducts

GenJet_Ver_II_vs_GenJet
Comparisonoftransfectionefficiency(leftpanel)andtoxicity(rightpanel)GenJet™Ver.IIwitholdversionGenJet™oncommonlyusedmammaliancells.phRL-CMVencodingRenillaLuciferasewasintroducedintocellswiththepresenceofserumandantibiotics.TheRenilliaLuciferaseactivitywasdeterminedwithRenillaLuciferaaseAssaySystem(Promega).Forcytotoxicity,MTTassaywasemployedtodeterminecellviabilitywithuntransfectedcontrolas100%.

GenJet_Ver_II_vs_L2K
AcomparisonoftransfectionefficiencyofGenJet™(Ver.II)vs.Lipofectamine2000onprimaryrataorticsmoothmusclecells.PrimaryrataorticsmoothmusclecellswerepreparedandtransfectedwithanucleartargetedGFPCDNAbyGenJet™(rightpanel)andLipofectamine2000(L2K,leftpanel)respectively.ThetransfectionefficiencywerecheckedunderaNikonEclipse2000fluorescencemicroscopy48hoursposttransfection

GenJet_Ver_II_vs_GenJet_HEK293
GenJet_Ver_II_vs_GenJet_HELA
UpperPanel:TransfectionefficiencycomparisonofGenJet™Ver.IIwitholdversionGenJet™on293Tcells.pEGFP-N3wastransfectedto293Tcellsat90%confluencywithpresenceofserumandantibiotics.GFPfluorescencewasdetectedwithNikonEclipse2000microscopy48hoursposttransfection
Lowerpanel:TransfectionefficiencycomparisonofGenJet™Ver.IIwitholdversionGenJet™onHelacells.pEGFP-N3wastransfectedtoHelacellsat90%confluencywithpresenceofserumandantibiotics.GFPfluorescencewasdetectedwithNikonEclipse2000microscopy48hoursposttransfection


TechnicalInformation&Datasheet
-ACompleteTransfectionProtocol
-AShortProtocolforAdvancedUsers
-AProtocolforTransfectingSUSPension293andCHOCells
AProtocolforLentivirusProduction
AProtocolrAAVProduction

-TechnicalNote&TransfectionTips


RelatedProduct:GenJet™InVitroDNATransfectionreagent

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品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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