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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/GenJet™ In Vitro DNA Transfection Reagent for MCF 10A Cell/SL100489-MCF10A/0.1 mL
产品编号:SL100489-MCF10A-5x1.0mL
市  场 价:¥20720.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1036.00
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/GenJet™ In Vitro DNA Transfection Reagent for MCF 10A Cell/SL100489-MCF10A/0.1 mL
商品介绍

Description
GenJet™DNAInVitroTransfectionReagentforMCF10ACellispre-optimizedfortransfectingthenon-tumOrigenicepithelialcellline,MCF10A.TheMCF10Acelllineisanon-tumorigenicepithelialcellline.Thecellsarepositiveforepithelialsialomucins,cytokeratinsandmilkfatglobuleantigen. Theyexhibitthreedimensionalgrowthincollagen,andformdomesinconfluentcultures.Thusfar,thecellshaveshownnosignsofterminaldifferentiationorsenescence. Thelineisresponsivetoinsulin,glucocorticoids,choleraenterotoxin,andepidermalgrowthfactor(EGF). Byelectronmicroscopythecellsdisplaycharacteristicsofluminalductalcellsbutnotofmyoepithelialcells.Withourproprietarytransfectionboosterconjugationtechnology,thisreagentwasformulatedtotransfectMCF10Acellswithupto50%efficiency(GFPpositivecells).
 

SummaryofOptimalShavedCellTransfectionConditions:
Cellculturemedium
Confluencyofcellonthedayofshaving
Celldensityforplating

GenJet™(µl):DNA(µg)Ratio
DiluentforDNAandTransfectionReagent

IncubationTimetoFormGenJet™/DNAComplex
Incubationtimeofshavedcellswithtransfectioncomplex
MaximalEfficiency

TransfectionResults:
ReporterGene
Plasmid

Efficiency(GFP%)


DMEM/F12with5%HSandotheradditives
95~100%
1.3x10^5cellspersquarec
entimeter
4:1
Serum-freeDMEM/F12

15minutesatRT
20minutesat37degree
48hours


EGFP
pEGFP-N3(CMVpromoter)

50%

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger

DataSheet

Torequestafreetrialsample,pleaseCreateAnAccountwithustoenteryourshippingaddressandemailusatorder@Signagen.com

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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