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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/GenJet™ In Vitro DNA Transfection Reagent for Neuro-2A Cells/SL100489-N2A/0.5 mL
产品编号:SL100489-N2A-5x1.0mL
市  场 价:¥15760.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$788.00
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/GenJet™ In Vitro DNA Transfection Reagent for Neuro-2A Cells/SL100489-N2A/0.5 mL
商品介绍

Description

GenJet™ DNA In Vitro Transfection Reagent for Neuro2A is pre-optimized for transfecting Neuro2A cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Neuro2A cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium 5 Hours After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~90%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes
48 hours


EGFP
pEGFP-N3 (CMV promoter)

89%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


A Picture Showing Transfection Efficiency of GenJet™ Reagent on Neuro2A Cells.  Neuro2A cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™ and visualized by a Nikon Eclipse Fluorescence microscope with DIC phase (left panel) and FITC (right panel) imaging 36 hours post transfection

Data Sheet

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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