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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/GenJet™ In Vitro DNA Transfection Reagent for RAW 264.7 Cell/SL100489-RAW/0.5 mL
产品编号:SL100489-RAW-5x1.0mL
市  场 价:¥35060.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1753.00
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/GenJet™ In Vitro DNA Transfection Reagent for RAW 264.7 Cell/SL100489-RAW/0.5 mL
商品介绍

Description

GenJet™ DNA In Vitro Transfection Reagent for RAW 264.7 Cell is pre-optimized for transfecting RAW 264.7 cells.  RAW 264.7 cells are a macrophage-like, Abelson leukemia virus transformed cell line derived from BALB/c mice. RAW 264.7 cell slide is often used for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

 

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)


~80%
DMEM with 10% FBS
3:1
1xGenJet™ Transfection Buffer
10 minutes at RT
Yes
No
48 hours


EGFP
pEGFP-N3 (CMV promoter)
75%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet & Protocol

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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