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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/PolyJet™ In Vitro DNA Transfection Reagent/SL100688/0.1 mL
产品编号:SL100688-5x1.0mL
市  场 价:¥13100.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$655.00
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/PolyJet™ In Vitro DNA Transfection Reagent/SL100688/0.1 mL
商品介绍
PolyJet™DNAInVitroTransfectionReagent

Description
Basedonourproprietarypolymersynthesistechnology,PolyJet™DNAInVitroTransfectionReagentisformulatedasabiodegradablepolymerbasedDNAtransfectionreagentthatensureseffectiveandreproducIBLetransfectiononHEK293,
COS-7,NIH-3T3,HeLa,CHOandabroadrangesofhard-to-transfectmammaliancells.PolyJet™reagentisabletoimmobilizeDNAmigrationduringelectrophoresisatverylowconcentrationandformtransfectioncomplexwithin5minutesatRT.Aremarkablefeatureofthereagentistherapidandcompletedegradationofpolymeraftertransfectioncomplexendocytosis(Figure1),leADIngtomuchlesscytotoxicity.PolyJet™reagent,1.0ml,issufficientfor~667transfectionsin24wellplatesor~333transfectionsin6wellplates,providingaveryaffordablealternativetotheleadingproductsfortransfectingavarietyofcommonlyusedandhard-to-transfectmammaliancells.


Figure1.ACartoonShowingBiodegradationofPolyJet™DNATransfectionReagentAfterEndocytosisofTransfectionComplex

Features
-Bio-degradableafterendocytosis
-Exceptionalhightitersofvirusproduction
-EquallygoodforverylongDNAs(>89kb)

-EquallygoodforbothsingleDNAtransfectionandmultiDNAco-transfection
-Highlevelsofrecombinantproteinproduction
-Simple&robusttransfectionprocedure
-Veryaffordable

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.

BroadTransfectionSpectrumforMammalianCellTypes

CellLines

Efficiency(%GFP)

CellLines

Efficiency(%GFP)

McArdle7777
Hep3D
SHEP
3T3-442A
COS-7
CV-1
D407
DHDPro.b
3LL
B16-F10
BAEC
BHK-21
CaSki
CaCo2
CHO
HCS-2/8
HEK-293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20
SK-N-SH
C2C12
HepG2

65-70%
67-76%
68-71%
35%
85-90%
60%
70%
70%
80%
85%
51%
80%
88%
60%
88%
61%
86%
88%
72%
59%
72%
46%
29%
46%
72%

hESCs
SN56

MC3T3-E1
Primarymelanocyte
mESCs
L929
MCF-7
MDCK
Neuro2A
NIH3T3
PC12
SH-SY5Y
SiHa
SKOV3
Huh-7
IGROV1
DF-1,ChickenEmbryonicCell
6CSFMEo
WEHI231
A549
LNCap
Prim.mousekeratinocyte
Prim.humanskinfibroblast
Prim.humanpre-adipocyte
Prim.mouseembry.fibroblast

70%
81%
80%
35%
70%
59%
68%
68%
86%
76%
50%
25%
60%
65%
70%
35%
50%
71%
26%
75%
75
29%

50%
32%
30%


ExamplesShowingTransfectionEfficiencyofPolyJet™DNAInVitroTransfectionReagentonCommonlyUsedCells
PolyJet_L2K_CHO
TransfectionefficiencycomparisonofPolyJet™vs.lipofectaminePlusonChineseHamsterOvary(CHO)cells.HAtaggedbeta-tubulinCDNAwasdeliveredintoCHOcellswithPolyJet™(leftpanel)andlipofectaminePlus(rightpanel)respectively.FITCconjugatedantibodyagainstHAtagwasutilizedtopickupHA-beta-tubulin(Green)whileaDM1aantibodywasusedtodetectendogenousalpha-tubulinfollowedbyprobingwithrhodamineconjugatedsecondaryantibody(Red).TheabovepicturewasprovidedbyDr.ShangYinofUniversityofTexasatHoustonMedicalSchoolascourtesy

PolyJet_L2K_HEK293
AcomparisonshowingtransfectionefficiencyofPolyJet™reagentvs.aleadingproduct,Lipofectamine2000onHEK293FTcells.HEK-293FTcellsweretransfectedwithGFPvector(pEGFP-N3)byPolyJet™(leftpanel)andLipofectamine2000(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection

PolyJet_L2K_HepG2
AcomparisonshowingtransfectionefficiencyofPolyJet™reagentvs.aleadingproduct,Lipofectamine2000onHepG2cells.HepG2cellsweretransfectedwithGFPvector(pEGFP-N3)byPolyJet™(leftpanel)andLipofectamine2000(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection

PolyJet_Fugene_HD_MDCK
TransfectionefficiencycomparisonofPolyJet™vs.FugeneHDonMDCKcells.AplainGFPDNAwastransducedintoMDCKcellswithPolyJet™(leftpanel)andFugeneHD(rightpanel)reagentsrespectivelypermanufacturers"protocols.GFPandDAPIstainingwerevisualizedunderfluorescencemicroscopy48hoursposttansfection.TheabovecomparisondataandpictureswerecompletedandprovidedbyDr.GeZhouofNYUMedicalCenterascourtesy

PolyJet_L2K_MDCK
AcomparisonshowingtransfectionefficiencyofPolyJet™reagentvs.aleadingproduct,Lipofectamine2000onMDCKcells.MDCKcellsarenotoriouslyhardtotransfect.Withproprietary"ShavedCellTransfection"protocol,PolyJet™(leftpanel)givesupto70%GFPpositivecellsvs.Lipofectamine2000(rightpanel)around5%efficiency.MDCKcellsweretransfectedwithGFPvector(pEGFP-N3)byPolyJet™(leftpanel)andLipofectamine2000(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope36hoursposttransfection

PolyJet_Fugene_HD_LNCap
AcomparisonshowingtransfectionefficiencyofPolyJet™reagentvs.aleadingproduct,FugeneHDonLNCapcells.LNCapcellsweretransfectedwithGFPvector(pEGFP-N3)byPolyJet™(leftpanel)andFugeneHD(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection

PolyJet_hESCs
AimageshowingexceptionaltransfectionefficiencyofPolyJet™reagentonHumanembryonicstemcells (hESCs). ThehESCsgrowninE8mediumonGeltrexvs(leftpanel,DICimaging)wastransfectedwithpEF1α-GFP.TheGFPexpression(rightpanel)wasvisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection. TheabovepictureswereprovidedbyDr.MarinaPryzhkovaofJohnsHopkinsUniversityascourtesy

PolyJet_L2K_N2A
Neuro2AcellstransfectedwithpEGFP-C1plasmidusingPolyJet™InVitroDNATransfectionReagent.TheNeuro2AcellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging(left)andFITCimaging(right)24hourspost-transfection

PolyJet_L2K_Primary_Fibroblast
ComparisonofcytotoxicityofPolyJet™DNAInVitroTransfectionReagentwithL2K™onprimarymurineskinfibroblast.Theprimarymurinefibroblastwasincubatedwiththeindicatedtransfectionreagents/pEGFP-C1(DNA)complexesabovefor4hoursinserum-freeDMEMHighGlucosemediumfollowedbyreplacementofcompleteserum-containingmedium.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging24hoursposttransfection

DataSheetandProtocols
-GeneralProtocolforTransfectingMammalianCells

-AShortProtocolforTransfectingMammalianCells
-AdvancedProtocolforTransfectingHard-to-TransfectMammalianCells

-AProtocolforTransfectingSUSPension293andCHOCells
-ProtocolforLentivirusProduction
-
ProtocolforrAAVProduction
TechnicalNote&TransfectionTips


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Testimonials:


Sorryforsooobigdelay,butnowIamtotallyinlovewithPolyJet.IhaveusedPolyJetforawhileonmouseESCswithrelativelygoodefficiency50~70%.

--------MarinaPryzhkova,Ph.D.,JHU


PolyJetTransfectionReagentworkedequallyaswellaslipofectamine2000,withlittleevidenceofcelldeathon293,PC-3and22RV1cells.Iwilldefiantlyconsiderswitchingover.

-----TiffanyWallace,Ph.D.,NCI/NIH


Ionlydidsidebysidewiththetestingsample(PolyJet)andLipo2000withGFPtransfectiononCOS-7cells.Theresultwasverygood.PolyJetwasevenbetterthanL2K.

------FengQiao,Ph.D.,NEI/NIH


ItestedthesampleofPolyJetonmyNIH-3T3mousefibroblaststhisweekend.TheresultsweremuchbetterthanLipofecatmineLTX.I"mattachingapowerpointslidewithmyresults(Ididnotquantifythe%transfectionefficiency,butthepicturesgetthepointacross).Ifoundthattheprotocolfordifficult-to-transfectcelllinesworkedbetterthanthestandardprotocol.

-----StephanieMurphy,Ph.D.,DartmouthCollege


Ihadchancetotryyourproductfinally.Itwasgreatsuccess.IusedHeLacellsandgot10%transfectionefficiency(<0.1%forLipofectamine).Thankyou!IwaswonderingifIalsotryGenJet™PlusDNAInVitroTransfectionReagent?Accordingtoyourwebsite,thereagentworksbetterthanregularPolyJet.

-------YumiUetake,Ph.D.,UMASS


ItestedPolyJetanditlooksgreatonMDCK.Weplacedorder.Thankyou!

-------GeZhou,Ph.D.,NYU


Wearehappytoprovidefeedback.PolyJetworkedverywellforusinHepG2cells,wegotapproximately80%efficiencywithpMAXGFPplasmid,byfollowingtheconditionsinyoursuggestedprotocol.WeranacomparisonwithLipofectamine,whichonlyshowedapproximately20-30%transfectionefficiency.Weareplanningexperimentsandwillbeorderingmoresoon.

-------EmilyMcallister,PBRC

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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