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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/rAAV Production Full Service, Large Scale/SL100801/
产品编号:SL100801
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/rAAV Production Full Service, Large Scale/SL100801/
商品介绍
rAAVProductionFullService,LargeScale

Features:
-Nonpathogenicwithleastimmuneresponseandbestsuitableforinvivoapplication.
-Excellentgenedeliveryefficiencyinmostcelltypesincludingdividingandnon-dividingorprimarycells.
-MultipleSEROtypes
(AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-8,AAV-9,AAV-DJ/8andAAV-DJ).
-Persistenttransgeneexpression.


Large AAVcisvectorinventory forconstructingAAVcisplasmidwith aspecificpromoterandreporter:

 SinglepromoterrAAVcisvector

 DualpromoterrAAVcisvector

         Promoterless
         CMV
         CAG
         H1
         
U6
         Synapsin
         UBC

         EF1α
         CBh
         
TRE-miniCMV
         CMVtight

         ALB(1.4)

         MHCK7
         ApoE/AAT1

         CaMKII
         ELA1
         Enh358MCK
         cTNT
         GFAP
         MBP
         SST
         TBG

         αMHC
         hRPE
         mIP1
         tMCK





















 
 

          Promoterless
          CMV
          CAG
          H1
          
U6
          Synapsin
          UBC

          EF1α
          CBh

          
TRE-miniCMV
          CMVtight

          ALB(1.4)

          MHCK7

          ApoE/AAT1
          CaMKII
          ELA1
          Enh358MCK
          cTNT
          GFAP
          MBP
          SST
          TBG

          αMHC
          hRPE
          mIP1
          tMCK

CMV
CAG
H1

U6
Synapsin
UBC

EF1α
CaMKII
cTNT
GFAP
TRE-miniCMV
CMVtight
MHCK7










 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ
TRE-miniCMV
CMVtight
MHCK7

 


ServiceDescription:
-Cloningyourgeneofinterest(GOI)intoAAVcisvector.
-rAAVcisplasmidlargeprep.
-rAAVpackaging
in2xcellstack.
-rAAVpurificationviaadvanced2xCsClultra-centrifugation.
-
Desalting,filtersterilizationandAAVtitrationviaqPCR.

rAAVSerotypesWeOffer:
AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-7,AAV-8,AAV-9,AAV-PHP.B,AAV-DJ/8andAAV-DJ.

RequiredMaterials:ANCBIaccession#orgenesymbolorplasmidDNAcarryingyourgeneofinterest(GOI).

TurnaroundTime:~3weeks.

Deliverables:>1.0mL(10x0.1mL)ofsuperpurifiedinvivograderAAVvectorat>1E+13VG/mL*.

Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.

ServiceInquiry/RequestaQuote

 

SuperHighYield:
WithourproprietarygeneticallyengineeredAAV·HT™packagingcellandaadvancedpurificationapproach,therAAVyieldiseasytoreachsuperhighlevel--------total1E+15VG.ForsuperhighlevelrAAVproductionupto1E+15VGtotal,pleasecontactustorequestaquote.
 


Figure1.AcomparisonofpurityandinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedandsuperinfectious(closetoclinicaltrialgrade)rAAVvectorpreparedviaouradvanceddoubleCsClultra-centrifugationapproach. 
A.rAAVvectors(total1E+9VGperlane) fromdifferentsourceswereresolvedonSDS-PAGEfollowedbysilverstaining. Lane1:GMPmanufacturedrAAVvectorfromCHOP;Lane2:rAAVpreparedviaouradvanced2xCsClultra-centrifugationapproach;Lane3:rAAVfromVectorCoreofBCM;Lane4:rAAVfromourcompetitor"V";Lane5:rAAVfromourcompetitor"C";Lane6:ProteinMarker.
B.SuperinfectiousrAAVvectorpreparedviaadvanceddoubleCsClultra-centrifugation. Leftpanel:rAAV9-GFP(total5E+9VG)fromourcompetitor"V"injectedtomouseeye;Rightpanel:rAAV-9-GFP(total5E+9VG)purifiedviaadvanced2xCsClultra-centrifugationinjectedtomouseeye. 



Figure2.AcomparisonofinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedrAAVpreparedviaadvanceddoubleCsClultra-centrifugationapproachissuperinfectious.
rAAV1-GFP(total2E+9VG)fromdifferentsourceswereinjectedtomousemuscletissue. TheGFPfluorescencewasvisualized3weekspostinjection. A.rAAV1-GFPfromourcompetitor"V";B.rAAV1-GFPfromourpre-maderAAVsstockpurifiedviaadvanceddoubleCsClultra-centrifugation. C.QuantificationdatashowedthatoursuperpurifiedrAAV(bar2)is~9timesmoreinfectiousthanthat(bar1)preparedviaconventionalCsClultra-centrifugation.  


*Finalviralyieldmaydependonthenatureoftransgene.

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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