商品介绍
-Excellentgenedeliveryefficiencyinmostcelltypesincludingdividingandnon-dividingorprimarycells.
-MultipleSEROtypes(AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-8,AAV-9,AAV-DJ/8andAAV-DJ).
-Persistenttransgeneexpression.
-Induceefficientgenesilencing.
ServiceDescription:
-Mediumscaleco-transfectionofAAV·HT293cellsinto1xcellstack.
-HarvestrAAVfollowedbypurificationviacolumnfiltrationand2xCsClultra-centrifugation.
-TitrationviaqPCR(measuringgenomecopies).
rAAVSerotypesWeOffer:
AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-7,AAV-8,AAV-9,AAV-PHP.B,AAV-DJ/8andAAV-DJ.
RequiredMaterials:
AAVcisplasmidcarryingyourshRNAorpre-miRNA,>150µg.
TurnaroundTime:
1 ~2weeks.
Deliverables:>0.2mLofsuperpurifiedinvivograderAAVvectorat>1E+13VG/mL*.
Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.
WealsooffertruncatedcustomshRNAAAVservice,pleaserequestaquotewithustoday.
Figure1.AcomparisonofpurityandinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedandsuperinfectious(closetoclinicaltrialgrade)rAAVvectorpreparedviaouradvanceddoubleCsClultra-centrifugationapproach.
A.rAAVvectors(total1E+9VGperlane) fromdifferentsourceswereresolvedonSDS-PAGEfollowedbysilverstaining. Lane1:GMPmanufacturedrAAVvectorfromCHOP;Lane2:rAAVpreparedviaouradvanced2xCsClultra-centrifugationapproach;Lane3:rAAVfromVectorCoreofBCM;Lane4:rAAVfromourcompetitor"V";Lane5:rAAVfromourcompetitor"C";Lane6:ProteinMarker.
B.SuperinfectiousrAAVvectorpreparedviaadvanceddoubleCsClultra-centrifugation. Leftpanel:rAAV9-GFP(total5E+9VG)fromourcompetitor"V"injectedtomouseeye;Rightpanel:rAAV-9-GFP(total5E+9VG)purifiedviaadvanced2xCsClultra-centrifugationinjectedtomouseeye.
Figure2.AcomparisonofinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedrAAVpreparedviaadvanceddoubleCsClultra-centrifugationapproachissuperinfectious.
rAAV1-GFP(total2E+9VG)fromdifferentsourceswereinjectedtomousemuscletissue. TheGFPfluorescencewasvisualized3weekspostinjection. A.rAAV1-GFPfromourcompetitor"V";B.rAAV1-GFPfromourpre-maderAAVsstockpurifiedviaadvanceddoubleCsClultra-centrifugation. C.QuantificationdatashowedthatoursuperpurifiedrAAV(bar2)is~9timesmoreinfectiousthanthat(bar1)preparedviaconventionalCsClultra-centrifugation.
*Finalviralyieldmaydependonthenatureoftransgene.
品牌介绍
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。
公司介绍
产品分类
G-protein Coupled Receptors
Transcription Factors
Adenovirus Production Service
Lentivirus Production Service
Ion Channels
Pre-made AAVs
Free Offers
Endopeptidases & Exopeptidases
Membrane Receptors
Protein Kianses
Miscellaneous Ready to Package Adenoviruses
AAV Production Service
Cytokines
Tumor and Apoptosis
In Vivo Transfection Reagents
Clones for Signal Transduction
Protein Kinase, Dead Mutants or Dominant Negatives
Phosphoric Diester Hydrolases
In Vitro siRNA Transfection Reagents
Western Blotting Products
Transfection Accessories
Phosphoprotein Phosphatases
shRNA/miRNA Adenovirus Svc
shRNA/miRNA AAV Service
Pre-optimized Transfection Reagents
In Vitro DNA Transfection Reagents
Pre-made Adenovirus
Pre-made Lentivirus
Ligand-dependent Nuclear Receptors
Cytochrome P450
销售排行
厂家直采
全球直采 正品优价
正品保障
厂家直发 有线跟踪
正规清关
CIF100%正规报关，提供发票
及时交付
限时必达 不达必赔
总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
工作时间：周一至周五9:00~18:00
技术支持：
短信：18915418616
服务热线：
4000-520-616（全国）
☏ 0512-67156496（苏州）
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