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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/shRNA/miRNA/gRNA rAAV Production Full Service, Medium Scale/SL100871/
产品编号:SL100871
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/shRNA/miRNA/gRNA rAAV Production Full Service, Medium Scale/SL100871/
商品介绍
shRNA/miRNA/gRNArAAVProductionFullService,MediumScale

Features:
-Excellentgenedeliveryefficiencyinmostcelltypesincludingdividingandnon-dividingorprimarycells.
-MultipleSEROtypes
(AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-8,AAV-9,AAV-DJ/8andAAV-DJ).
-Persistenttransgeneexpression.
-Induceefficientgenesilencing.

LargeshRNA/miRNA/gRNAAAVcisvectorinventoryforconstructingAAVcisplasmidwithaspecificpromoterandreporter:

 
 ForConventionalshRNA/miRNA/TuDrAAVProduction

 Single-promotershRNA/miRNAAAVcisvector

 Dual-promotershRNA/miRNArAAVcisvector

            Promoterless
            CMV
            CAG
            H1
            
U6
            Synapsin
            UBC

            EF1α
            ALB(1.4)
            ApoE/AAT1
            CaMKII
            ELA1
            Enh358MCK
            cTNT
            GFAP
            MBP
            SST
            TBG

            αMHC
            hRPE
            mIP1
            tMCK





















 

            Promoterless
            CMV
            CAG
            H1
            
U6
            Synapsin
            UBC

            EF1α
            ALB(1.4)
            ApoE/AAT1
            CaMKII
            ELA1
            Enh358MCK
            cTNT
            GFAP
            MBP
            SST
            TBG

            αMHC
            hRPE
            mIP1
            tMCK

 CMV
 CAG
 H1
 
U6
 Synapsin
 UBC

 EF1α
 CaMKII
 cTNT
 GFAP











 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ











 

 
 ForCRISPR/Cas9rAAVProduction

 Single-promoterCRISPR/Cas9AAVcisvector

 Dual-promoterCRISPR/Cas9AAVcisvector

     Promoterless
      U6
      H1
     CMV
     CAG
     CBH
      Synapsin
      UBC

      EF1α
      ALB(1.4)
      ApoE/AAT1
      CaMKII
      ELA1
      Enh358MCK
      cTNT
      GFAP
      MBP
      SST
      TBG

      αMHC
      hRPE
      mIP1
      tMCK

 Empty
 CBH
 U6
  H1
 sCMV
 Syn
  sEF1α
 CK0.4
 cTNT
  GFAP0.6
 sSyn
  SST
  TBG
  αMHC
  hRPE
  mIP1
  tMCK
 CBH

Cas9
hCas9

SpCas9
hSpCas9
SaCas9
AsCpf1
hAsCpf1
ftCas9
st1Cas9
cjCas9
cdCas9
ciCas9
ncCas9
st3Cas9
nmCas9
mgCas9
nsCas9

 

     Promoterless
     sCMV
     U6
     H1
     tRNA
     Syn
     sSyn
     
sEF1α
     CaMKII0.4

 CMV
 sCMV
 CBH
 H1
 
U6
 Syn
 sSyn

 sEF1α
 CaMKII0.4





 

Cas9
hCas9

SpCas9
hSpCas9
SaCas9
AsCpf1
hAsCpf1
ftCas9
st1Cas9
cjCas9
cdCas9
ciCas9
ncCas9
st3Cas9
nmCas9
mgCas9
nsCas9


 


ServiceDescription:
1.SynthesizeandcloneshRNAintoAAVcisvectors.
2.Medium
scaletransfectionofAAV·HT™293cellinto1xcellstack.
3.HarvestrAAVparticlefollowedby
purificationviaadvanced2xCsClultra-centrifugationtoobtainclinicaltrialgradeofrAAVvector.
4.rAAVtitrationviaqPCR.

rAAVSerotypesWeOffer:
AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-7,AAV-8,AAV-9,AAV-PHP.B,AAV-DJ/8andAAV-DJ.

RequiredMaterials:NCBIaccession#,targetsequence,validatedsiRNAorvalidatedshRNAsequence;miRNAaccession#,pre-miRNA,maturemiRNAorTuD(toughdecoy)miRNA.

TurnaroundTime:3~4weeks.

Deliverables:
>0.2mLsuperpurifiedinvivogradeofrAAVvectorat>1E+13VG/mL*.

Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.

WealsooffertruncatedcustomshRNA/miRNAAAVservice,please
requestaquotewithustoday.
 


Figure1.AcomparisonofpurityandinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedandsuperinfectious(closetoclinicaltrialgrade)rAAVvectorpreparedviaouradvanceddoubleCsClultra-centrifugationapproach. 
A.rAAVvectors(total1E+9VGperlane) fromdifferentsourceswereresolvedonSDS-PAGEfollowedbysilverstaining. Lane1:GMPmanufacturedrAAVvectorfromCHOP;Lane2:rAAVpreparedviaouradvanced2xCsClultra-centrifugationapproach;Lane3:rAAVfromVectorCoreofBCM;Lane4:rAAVfromourcompetitor"V";Lane5:rAAVfromourcompetitor"C";Lane6:ProteinMarker.
B.SuperinfectiousrAAVvectorpreparedviaadvanceddoubleCsClultra-centrifugation. Leftpanel:rAAV9-GFP(total5E+9VG)fromourcompetitor"V"injectedtomouseeye;Rightpanel:rAAV-9-GFP(total5E+9VG)purifiedviaadvanced2xCsClultra-centrifugationinjectedtomouseeye. 



Figure2.AcomparisonofinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedrAAVpreparedviaadvanceddoubleCsClultra-centrifugationapproachissuperinfectious.
rAAV1-GFP(total2E+9VG)fromdifferentsourceswereinjectedtomousemuscletissue. TheGFPfluorescencewasvisualized3weekspostinjection. A.rAAV1-GFPfromourcompetitor"V";B.rAAV1-GFPfromourpre-maderAAVsstockpurifiedviaadvanceddoubleCsClultra-centrifugation. C.QuantificationdatashowedthatoursuperpurifiedrAAV(bar2)is~9timesmoreinfectiousthanthat(bar1)preparedviaconventionalCsClultra-centrifugation.  


*Finalviralyieldmaydependonthenatureoftransgene.  
 

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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