Description
GenJetPlusDNAInVitroTransfectionReagentisenhancedversionofGenJetDNAInVitroTransfectionReagent.GenJetPlusisformulatedbyadditionofaboosterpeptide,givingrisetoupto20timeshigherefficiencyonvarietyofmammaliancellsincomparisonofitspreviousversion.GenJetPluswasshowntoefficientlydelivergenestovariousestablishedcelllinesaswellasprimarycellsincludingHEK293,293T,293E,CHO,COS1,HeLa,NIH3T3,insectcelllines(Sf9andSf21)andavarietyofothereucaryoticcelllineswithlesstoxicity.GenJetPlusreagent,1.0ml,issufficientfor667transfectionsin24wellplatesor333transfectionsin6wellplates.
FeaturescomparedwitholdversionGenJetInVitroDNATransfectionReagent
-Cell-dependent3~20timeshigherefficiency
-Topchoiceforhard-to-transfectcells
-EquallygoodforverylongDNAs(upto46kb)
-Efficiencyboostedinthepresenceofserumandantibioticsformostofcelltypes
-Exceptionalhighlevelsofrecombinantproteinproduction
StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.
ComparisonsofTransfectionEfficiencyofGenJetPlusInVitroDNATransfectionReagentwiththeLeADIngBrandNameProducts
GenJetPlusistopchoiceforhard-to-transfectcells.EfficiencycomparisonofGenJetPlusInVitroDNATransfectionReagent(leftpanel)withLipofectamine2000(L2K,rightpanel)onprimaryculturedrataorticsmoothmusclecells.AnucleartargetedGFPDNAwastransfectedwithGenJetPlusandL2Krespectivelypermanufacturer"sprotocols.Theefficiencywaschecked48hoursposttransfection.TheabovepictureswerekindlyprovidedbyDr.NickolaiDulinofSectionofPulmonaryandCriticalCare,UniversityofChicago
LeftPanel:ComparisonofGenJetPlusInVitroDNATransfectionReagentwithleadingproductsincludingLipofectamine2000(L2K)andLipofectamineLTX(LTX)onlentivirusproductionon293Tcells.ThedatawereprovidedbyDr.MiguelToscanoofTempleUniversityascourtesy.
Rightpanel:ComparisonofGenJetPlusInVitroDNATransfectionReagentwithtransfectionreagentsfromothervendorsinludingLipofectamine2000(L2K)andFuGene6onHelacells.HeLacellsweretransfectedwithplasmidDNAencodingGFP.Transfectionswereperformedaccordingtomanufacturersrecommendationsandanalysedbyflowcytometry.
Exceptionalhightitersoflentivirusgeneratedfrom293Tcells.ThreeDNAsco-transfectedwithGenJetPlusInVitroDNATransfectionReagent(leftpanel)incomparisonofLipofectamineLTX(rightpanel).1.0and10microlitersvirussupernatantfrom293Tcellswereusedtocounter-infected293FcellswhichwasthenpassedFACS.Thenumbersattheupperrightcornerindicatethepercentageoftransducedcells.ThetitersoflentivirusgeneratedwithGenJetPlusandLipofectamineLTXwerequantifiedtobe2.1x106and9.3x106tu/mlrespectively.TheabovedatawereprovidedbyDr.MiguelToscanoofTempleUniversityascourtesy.
TechnicalInformation&Datasheet
-GenJetPlusDataSheet&GeneralProtocol
-GenJetPlusInVitroDNATransfectionforLentivirusProduction
-GenJetPlusInVitroDNATransfectionforrAAVProduction
-TransfectionofSf9InsectCellswithGenJet
RelatedProduct:GenJetInVitroDNATransfectionreagent
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606),SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。