ProductCategory:Pre-maderecombinantAAV;AAV-CAG-GCaMP6f;AAV-CBA-GCaMP6f;AAV1-CAG-GCaMP6f;AAV1-CBA-GCaMP6f
Description:
GCaMPisageneticallyencodedcalciumindicator(GECI)andcreatedfromafusionofgreenfluorescentprotein(GFP),calmodulin(CaM),andM13,apeptidesequencefrommyosinlightchainkinase. WhenGECIbindsCa2+,theconformationalchangeofGCaMP inducesproximityofthe GFPparts,eliciting strongGFPfluorescencesignal,whichallowsmeasuringactionpotentialsandotherreceptoractivationeventsthattriggerCa2+influx.TheadvantageofGECI"sarethattheycanbegeneticallyspecifiedforstudiesinlivingorganisms.
GCaMP6isanovelultra-sensitiveformatofGCaMPthatoutperformedothersensorsintermsofaccuracyandsensitivityinmeasuringcytosolCa2+level. MutationsincalmodulinpartofGCaMP6createdseveralvariants whichshoweddifferentcalciumfluorescencedecayrate(fast- GCamP6f, slow -GCaMP6f andmedium-GCaMP6m).
TheAAV1-CAG-GCaMP6fisapre-packagedrAAVinSEROtype1(withcapsidfromAAVserotype1and2xITRfromAAVserotype2)whichover-expressesgeneticallyencodedcalciumindicatorGCaMP6f underCAGpromoter(alsoknownasCBApromoter).
Titer: >1x1013 VG/mL
Size: >30 µL(StdPack)
StorageBuffer: PBS
InfectionProtocol:
VectorMap:
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