Signagen/BlotFresh Western Blot Stripping Reagent (Ver. II)/SL100324/500 mL,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/BlotFresh Western Blot Stripping Reagent (Ver. II)/SL100324/500 mL

产品编号：SL100324-500mL

市场价：¥2160.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞凋亡>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$108.00

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

Application:
StripWesternBlotforforre-probingandformassspectrometry

Features:
-Stripblotin5~15minutes
-Incubationatroomtemperature
-Nostenchsmellingwithoutadditionof2-mercaptoethanoloritsanalogs
-Aproprietyformulationwithaneco-friendlyandnon-hazardousorganicsolvent(withlightodor)includedtocompletelydisassociatetheboundantibodies

ApplicationI.StripWesternBlotforRe-probing
AComparisonofBlotFreshStrippingReagentwithPierce"sRestoreBlotStrippingBuffer

-BlotStrippedandRe-probedwiththeSameAntibody*:

TotalAkt(catalog#A0501-1)withdifferenttreatmentsinNIH3T3cells
*Develop2minuteswithECLPlusandexpose20secondsonKodaknegatives

-BlotFreshStrippingReagentisMoreEffectiveinBreakingDowntheAntigen-AntibodyComplexthanPierce"sRestoreStrippingBuffer:

BlotFreshStrippingReagent*Pierce"sRestoreStrippingBuffer*
Original Exposex1" Exposex5" Original Exposex1" Exposex5"

*Theblotswereinitiallyprobedwithβ-actinantibodywithexposurefor10seconds.ThentheblotswerestrippedwithBlotFreshStrippingReagentfor5minutesandwithPierce"sRestoreStrippingBufferfor15minutesrespectively.Afterwashes,thestrippingeffectwastestedbydevelopmentofblotswithECLPlusfor2minutesfollowedbyexposuretoKodakBioMaxfilmsfor1and5minutesrespectively

-BlotFreshStrippingReagentStripsaBlotfollowedbyRe-probingwithaDifferentAntibody:

A.BlotwasprobedwithantibodyagainsttotalAkt(catalog#A0501-1)*.
B.BlotwasstrippedwithBlotFreshStrippingReagentandre-probewithantibodyagainstphospho-Akt(catalog#A0503-1)*.
*Lane1isserumstarvedovernightwhilelane2~5weretreatedwithPDGFindifferentconcentrationsinNIH3T3cells.Theblotsweredeveloped2minuteswithECLPlusreagentandexpose10secondsonKodaknegatives(ECLPlusisatrademarkofGEHealthcare-Amersham)

ApplicationII.StripWesternBlotforMassSpectrometry
ProceduresforStrippingWesternBlotforMassSpectrometry:
-Stripblotfor15minutesatRTtoremoveprimaryandsecondaryantibodies
-Locatetheproteinbycomparingthestrippedblotandexposedfilmandcutthebandfromblot
-Digestthecutbandfollowedbymassspectrometry(seethefollowingpicture)

DataSheet

Testimonials:
...hadreceivedsampleofBlotFreshWesternBlotStrippingReagent.Fantasticproduct!!Haveusedtostripsameblot2x,andresultingthreerADIographsareindistinguishablefromprimaryblotswiththesecondtwoantibodies.I"llbeorderingsometoday...
------byDr.CatherineBair,NCI,NIH

Icannotlivewithoutit!Itisjustamazing.IusedtousePiercesandIoftenneedtore-runWesternduetodirtyblotafterstrip.SinceIstartedusingthisproduct,mylifehasbeenchangedItmakesmyresearcheasierandIdonotneedtore-rungelanymoreAndthestrippingprocessisjust2minutes.Itisreallyamazingproduct!
------byDr.JenniferLin,theGeorgeWashingtonUniversity

SomeProductCitations:
1.MahmoudM.El-Mas,MingFan,andABDelA.Abdel-Rahman.Endotoxemia-MediatedInductionofCardiacInducIBLeNitric-OxideSynthaseExpressionAccountsfortheHypotensiveEffectofEthanolinFemaleRats.J.Pharmacol.Exp.Ther.2008;324:368-375
2.GrzegorzWoszczek,Li-YuanChen,SahrudayaNagineni,SaraAlsAATy,AnyaHarry,CaroleaLogun,RafalPawliczak,andJamesH.Shelhamer.IFN-gammaInducesCysteinylLeukotrieneReceptor2ExpressionandEnhancestheResponsivenessofHumanEndothelialCellstoCysteinylLeukotrienes.TheJournalofImmunology,2007;178:5262-5270
3.DavidABaker,AricMadayag,LarsVKristianse,JamesHMeador-Woodruf,VahramHaroutuniaandIlangovanRaju.ContributionofCystineGlutamateAntiporterstothePsychotomimeticEffectsofPhencyclidine.Neuropsychopharmacology,2007;Aug.29onlinepublication
4.AshleyB.WilliamsandPatriciaL.Foster.TheEscherichiacoliHistone-likeProteinHUHasaRoleinStationaryPhaseAdaptiveMutation.Genetics.2007;177:723735

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。