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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/BlotFresh™ Western Blot Stripping Reagent (Ver. II)/SL100324/125 mL
产品编号:SL100324-500mL
市  场 价:¥2160.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$108.00
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/BlotFresh™ Western Blot Stripping Reagent (Ver. II)/SL100324/125 mL
商品介绍

Application:
StripWesternBlotforforre-probingandformassspectrometry

Features:
-Stripblotin5~15minutes
-Incubationatroomtemperature
-Nostenchsmellingwithoutadditionof2-mercaptoethanoloritsanalogs
-Aproprietyformulationwithaneco-friendlyandnon-hazardousorganicsolvent(withlightodor)includedtocompletelydisassociatetheboundantibodies

ApplicationI.StripWesternBlotforRe-probing
AComparisonofBlotFresh™StrippingReagentwithPierce"sRestore™BlotStrippingBuffer

-BlotStrippedandRe-probedwiththeSameAntibody*:



TotalAkt(catalog#A0501-1)withdifferenttreatmentsinNIH3T3cells
*Develop2minuteswithECLPlus
™andexpose20secondsonKodaknegatives

-B
lotFresh™StrippingReagentisMoreEffectiveinBreakingDowntheAntigen-AntibodyComplexthanPierce"sRestore™StrippingBuffer:

BlotFresh™StrippingReagent*Pierce"sRestore™StrippingBuffer*
     Original          Exposex1"    Exposex5"         Original        Exposex1"    Exposex5"

*Theblotswereinitiallyprobedwithβ-actinantibodywithexposurefor10seconds.ThentheblotswerestrippedwithBlotFresh™StrippingReagentfor5minutesandwithPierce"sRestore™StrippingBufferfor15minutesrespectively.Afterwashes,thestrippingeffectwastestedbydevelopmentofblotswithECLPlus™for2minutesfollowedbyexposuretoKodakBioMaxfilmsfor1and5minutesrespectively

-BlotFresh™StrippingReagentStripsaBlotfollowedbyRe-probingwithaDifferentAntibody:


A.BlotwasprobedwithantibodyagainsttotalAkt(catalog#A0501-1)*.
B
.
BlotwasstrippedwithBlotFresh™StrippingReagentandre-probewithantibodyagainstphospho-Akt(catalog#A0503-1)*.
*
Lane1isserumstarvedovernightwhilelane2~5weretreatedwithPDGFindifferentconcentrationsinNIH3T3cells.Theblotsweredeveloped2minuteswithECLPlus™reagentandexpose10secondsonKodaknegatives(ECLPlus™isatrademarkofGEHealthcare-Amersham)

ApplicationII.StripWesternBlotforMassSpectrometry
ProceduresforStrippingWesternBlotforMassSpectrometry:
-Stripblotfor15minutesatRTtoremoveprimaryandsecondaryantibodies
-Locatetheproteinbycomparingthestrippedblotandexposedfilmandcutthebandfromblot
-Digestthecutbandfollowedbymassspectrometry(seethefollowingpicture)


DataSheet

Testimonials:
...hadreceivedsampleofBlotFresh™WesternBlotStrippingReagent.Fantasticproduct!!Haveusedtostripsameblot2x,andresultingthreerADIographsareindistinguishablefromprimaryblotswiththesecondtwoantibodies.I"llbeorderingsometoday...
------byDr.CatherineBair,NCI,NIH

Icannotlivewithoutit!Itisjustamazing.IusedtousePierce’sandIoftenneedtore-runWesternduetodirtyblotafterstrip.SinceIstartedusingthisproduct,mylifehasbeenchanged…ItmakesmyresearcheasierandIdonotneedtore-rungelanymore…Andthestrippingprocessisjust2minutes.Itisreallyamazingproduct!

------byDr.JenniferLin,theGeorgeWashingtonUniversity

SomeProductCitations:
1.MahmoudM.El-Mas,MingFan,andABDelA.Abdel-Rahman.Endotoxemia-MediatedInductionofCardiacInducIBLeNitric-OxideSynthaseExpressionAccountsfortheHypotensiveEffectofEthanolinFemaleRats.J.Pharmacol.Exp.Ther.2008;324:368-375
2.GrzegorzWoszczek,Li-YuanChen,SahrudayaNagineni,SaraAlsAATy,AnyaHarry,CaroleaLogun,RafalPawliczak,andJamesH.Shelhamer.IFN-gammaInducesCysteinylLeukotrieneReceptor2ExpressionandEnhancestheResponsivenessofHumanEndothelialCellstoCysteinylLeukotrienes.TheJournalofImmunology,2007;178:5262-5270
3.DavidABaker,AricMadayag,LarsVKristianse,JamesHMeador-Woodruf,VahramHaroutuniaandIlangovanRaju.ContributionofCystine–GlutamateAntiporterstothePsychotomimeticEffectsofPhencyclidine.Neuropsychopharmacology,2007;Aug.29onlinepublication
4.AshleyB.WilliamsandPatriciaL.Foster.TheEscherichiacoliHistone-likeProteinHUHasaRoleinStationaryPhaseAdaptiveMutation.Genetics.2007;177:723–735

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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