Description
CalFectinMammalianDNATranfectionReagentisapre-optimizedandrefinedversionoftrADItionalcalciumphosphatetransfectionreagent.Withanewchemistry,CalFectinReagentcontainsmuchlowerconcentrationofcalcium,leadingtomuchlowercytotoxicitywithoutsacrificingtransfectionefficiency.Inaddition,CalFectinReagentisaneffectivemethodfortheproductionoflong-termstableandtransienttransfectantsonmostadherentcelllines.comparedwithliposomeandpolymerbasedtransfectionreagents,CalFectinReagentshowsdistinguishedfeaturesandmuchhigherefficiencyonHepG2,HEK293,CHO,Hela,COS,3T3,LNcap,C6,PC12andprimaryculturedcells,etc.CalFectinReagent,1.0ml,issufficientfor~666transfectionsin24wellplatesor~333transfectionsin6wellplates.
Application
High-efficiencystableortransienttransfectionofeukaryoticcells.
Feature
-Highefficient:Componentsoptimizedforhightransfectionefficiencies
-Simpleprocedures:Totalonetubereaction
StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.
ComparisonsofTransfectionEfficiencyofCalFectinReagentwiththeLeadingBrandNameProduct
AcomparisonstudyshowingtransfectionefficiencyofCalFectinReagentvs.aleadingproduct,Lipofectamine2000onHEK293Tcells.HEK293TcellsweretransfectedwithGFPtaggedDNA(total14kb)byCalFectin(leftpanel)andLipofectamine2000(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection
AcomparisonstudyshowingtransfectionefficiencyofCalFectinReagentvs.aleadingproduct,Lipofectamine2000onHepG2cells.HepG2cellsweretransfectedwithGFPvector(pEGFP-N3)byCalFectin(leftpanel)andLipofectamine2000(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection
TechnicalInformation&Datasheet
-AGeneralProtocolforTransfectingMammalianCells
-AProtocolforLentivirusProduction
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