ProductionofRecombinantSEROtype5Adenovirus(E1/E3deletion)withAd.MAXSystem:
-Unsurpassabletransductionefficiency(nearly100%vs.AAV30~40%andLentivirus~30%)inmostmammaliancells.
-Nointegrationintohostgenome.
-CompatIBLewithtoxicgeneofinterest(GOI).
-Inducehigh-leveltransientproteinexpression.
-Accommodateinsertsofupto7.5kb.
LargecollectionofAd.MAX™shuttlevectorforcloningshuttleplasmid with aspecificpromoterandreporter:
SinglepromoterAd.MAX™shuttlevector | DualpromoterAd.MAX™shuttlevector | |||
Promoterless |
| Promoterless | Promoterless | eGFP |
ServiceDescription:
1.Cloneyourgeneofinterest(GOI)intoashuttlevectorfollowedbytransferofthewholetransgenecassettetoadenoviralgenome.
2.Initialadenoviralstockproduction.
3.Adenovirusamplificationfrom~500cm2Ad.MAX™293cells.
4.RecombinantadenovirusPFUtitration.
RequiredMaterial:ANCBIaccession#orgenesymbolorplasmidDNAcarryingyourgeneofinterest(GOI).
TurnaroundTime:3~4weeks.
Deliverables:>1.0mL(5x0.2mL)superinfectiousinvitrogradeofadenovirusvectorat5E+10~5E+11PFU/mL*.
Weofferdiscountfornewcustomers,pleaserequestaquotewithustoday.
Weoffertruncatedserviceaswell,pleaserequestaquotewithustoday.
*Finaladenovirusyieldmaydependonthenatureoftransgene.
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606),SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。