Signagen/GenJet In Vitro DNA Transfection Reagent/SL100488/1.0 mL,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/GenJet In Vitro DNA Transfection Reagent/SL100488/1.0 mL

产品编号：SL100488-5x1.0mL

市场价：¥14820.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$741.00

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

GenJetInVitroDNATransfectionReagent

Description

GenJetDNAInVitroTranfectionReagentisapowerfultransfectionreagentthatensureseffectiveandreproducIBLetransfectionwithinvisibletoxicity.GenJetisformulatedbyuniquechemistry(covalentlycross-linkingcationiclipidswithpolymer),givingrisetoexceptionaltransfectionefficiencywithdistinguishablefeaturesincomparisonofothertypesofreagents.GenJetwasshowntodelivergenestovariousestablishedcelllinesaswellasprimarycellsincludingHEK293,293T,293E,CHO,COS1,HeLa,NIH3T3,insectcelllines(Sf9andSf21)andavarietyofothereucaryoticcelllines.GenJetreagent,1.0ml,issufficientfor~666transfectionsin24wellplatesor~333transfectionsin6wellplates.

Application
-DeliverDNA/RNAintomammalian,insectcells
-Greatlyenhance(upto200timeshigherefficiency)virus(e.g.,replication-deficientAdenovirus)infectionofhard-to-transfectcells

Features
-Exceptionalhightitersofvirusproduction
-EquallygoodforverylongDNAs(upto56kb)
-EquallygoodforsingleormultipleDNAtransfections
-Exceptionalhighlevelsofrecombinantproteinproduction
-Topchoiceforhard-to-transfectcells
-Inexpensivetransfectionreagent

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.

BroadTransfectionSpectrumforMammalianCellTypes

CellLines

TransfectionEfficiency(%GFP)

CellLines

TransfectionEfficiency(%GFP)

3LL
B16-F10
BAEC
BHK-21
CaSki
CaCo-2
CHO
HCS-2/8
HEK-293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20
SK-N-SH
McArdle7777
Hep3D
SHEP
MDA-MB-231
3T3-442A
COS-7
CV-1
D407
DHDPro.b
LS180

51%
72%
52%
86%
78%
32%
83%
42%
80-90%
81%
50-65%
49%
22%
38%
20%
60%
78%
71%
38%
10%
60-70%
53%
62%
51%
46%

K562
L929
MCF-7
MDCK
Neuro2A
NIH3T3
PC12
SH-SY5Y
SiHa
SKOV3
HUVEC
IGROV1
Jurkat
6CSFMEo
WEHI231
SAOS-2
SN56
MC3T3-E1
BT474
C2C12
Primarymelanocyte
Hela-S3
A549
HepG2

22%
52%
51%
52%
72%
88%
34%
14%
62%
52%
51%
21%
5%-15%
73%
25%
52%
69%
70%
46%
46%
46%
78%
78%
58%

ComparisonsofTransfectionEfficiencyandCytotoxicityofGenJetDNAInVitroTransfectionReagentwiththeLeADIngBrandNameProducts

ExceptionaltransfectionefficiencyofextremelylongDNAbyGenJetDNAInVitroTransfectionReagent.HEK293cellsweretransfectedwithGFPtaggedDNA(total14kb)byGenJet(upperpanel)andFuGene6(lowerpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphase(leftpanel)andFITC(rightpanel)imaging24hoursposttransfection

Exceptionalhightitersoflentivirusgeneratedfrom293Fcells.ThreeDNAsco-transfectedwithGenJetInVitroDNATransfectionReagent(leftpanel)incomparisonofLipoFectamine2000(L2K,rightpanel).100microlitervirussupernatantfrom293Fcellswereusedtocounter-infected293FcellswhichwasthenpassedFACS.Thenumbersattheupperrightcornerindicatethepercentageoftransducedcells.ThetitersoflentivirusgeneratedwithGenJetandL2Kwerequantifiedtobe7.9x10⁶and3.1x10⁶tu/mlrespectively

PrimaryMurineSkinFibroblasttransfectedwithpEGFP-C1plasmidusingGenJetInVitroDNATransfectionReagent(upperpanel)andthemostpopularbrandproductFuGeneHD(lowerpanel).ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging(leftpanel)andFITCimaging(rightpanel)24hourspost-transfection

PrimaryMurineKeratinocytestransfectedwithpEGFP-C1plasmidusingGenJetInVitroDNATransfectionReagent(upperpanel)andoneofthemostpopularbrandproductFuGeneHD(lowerpanel).ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging(leftpanel)andFITCimaging(rightpanel)24hourspost-transfection

ComparisonofcytotoxicityofGenJetDNAInVitroTransfectionReagentwithL2Konprimarymurineskinfibroblast.Theprimarymurinefibroblastwasincubatedwiththeindicatedtransfectionreagents/pEGFP-C1(DNA)complexesabovefor4hoursinserum-freeDMEMHighGlucosemediumfollowedbyreplacementofcompleteserum-containingmedium.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging24hoursposttransfection

TechnicalInformation&Datasheet
-GenJetInVitroDNATransfectionDatasheet
-GenJetMSDS

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。