Description
GenJetDNAInVitroTranfectionReagentisapowerfultransfectionreagentthatensureseffectiveandreproducIBLetransfectionwithinvisibletoxicity.GenJetisformulatedbyuniquechemistry(covalentlycross-linkingcationiclipidswithpolymer),givingrisetoexceptionaltransfectionefficiencywithdistinguishablefeaturesincomparisonofothertypesofreagents.GenJetwasshowntodelivergenestovariousestablishedcelllinesaswellasprimarycellsincludingHEK293,293T,293E,CHO,COS1,HeLa,NIH3T3,insectcelllines(Sf9andSf21)andavarietyofothereucaryoticcelllines.GenJetreagent,1.0ml,issufficientfor~666transfectionsin24wellplatesor~333transfectionsin6wellplates.
Application
-DeliverDNA/RNAintomammalian,insectcells
-Greatlyenhance(upto200timeshigherefficiency)virus(e.g.,replication-deficientAdenovirus)infectionofhard-to-transfectcells
Features
-Exceptionalhightitersofvirusproduction
-EquallygoodforverylongDNAs(upto56kb)
-EquallygoodforsingleormultipleDNAtransfections
-Exceptionalhighlevelsofrecombinantproteinproduction
-Topchoiceforhard-to-transfectcells
-Inexpensivetransfectionreagent
StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.
BroadTransfectionSpectrumforMammalianCellTypes
CellLines | TransfectionEfficiency(%GFP) | CellLines | TransfectionEfficiency(%GFP) |
3LL | 51% | K562 | 22%
ExceptionaltransfectionefficiencyofextremelylongDNAbyGenJetDNAInVitroTransfectionReagent.HEK293cellsweretransfectedwithGFPtaggedDNA(total14kb)byGenJet(upperpanel)andFuGene6(lowerpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphase(leftpanel)andFITC(rightpanel)imaging24hoursposttransfection Exceptionalhightitersoflentivirusgeneratedfrom293Fcells.ThreeDNAsco-transfectedwithGenJetInVitroDNATransfectionReagent(leftpanel)incomparisonofLipoFectamine2000(L2K,rightpanel).100microlitervirussupernatantfrom293Fcellswereusedtocounter-infected293FcellswhichwasthenpassedFACS.Thenumbersattheupperrightcornerindicatethepercentageoftransducedcells.ThetitersoflentivirusgeneratedwithGenJetandL2Kwerequantifiedtobe7.9x106and3.1x106tu/mlrespectively PrimaryMurineSkinFibroblasttransfectedwithpEGFP-C1plasmidusingGenJetInVitroDNATransfectionReagent(upperpanel)andthemostpopularbrandproductFuGeneHD(lowerpanel).ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging(leftpanel)andFITCimaging(rightpanel)24hourspost-transfection PrimaryMurineKeratinocytestransfectedwithpEGFP-C1plasmidusingGenJetInVitroDNATransfectionReagent(upperpanel)andoneofthemostpopularbrandproductFuGeneHD(lowerpanel).ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging(leftpanel)andFITCimaging(rightpanel)24hourspost-transfection ComparisonofcytotoxicityofGenJetDNAInVitroTransfectionReagentwithL2Konprimarymurineskinfibroblast.Theprimarymurinefibroblastwasincubatedwiththeindicatedtransfectionreagents/pEGFP-C1(DNA)complexesabovefor4hoursinserum-freeDMEMHighGlucosemediumfollowedbyreplacementofcompleteserum-containingmedium.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging24hoursposttransfection
品牌介绍
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606),SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。
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