Signagen/GenJet In Vitro DNA Transfection Reagent (Ver. II)/SL100489/0.5 mL,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/GenJet In Vitro DNA Transfection Reagent (Ver. II)/SL100489/0.5 mL

产品编号：SL100489-5x1.0mL

市场价：¥15760.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$788.00

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

GenJetInVitroDNATransfectionReagent(VersionII)

Description
GenJetInVitroDNATransfectionReagent(Ver.II)isupgradedversionofGenJetInVitroDNATransfectionReagent.Adoptinganewchemistry,moreDNAcondensinggroupswereliberatedinGenJet(Ver.II)(100%vs.80%inoldversion),leADIngto3~4foldsincreaseoftransfectionefficiencywithoutsacrificingcellviABIlity.GenJet(Ver.II)reagent,1.0ml,issufficientfor~667transfectionsin24wellplatesor~333transfectionsin6wellplates.

FeaturescomparedwitholdversionGenJetInVitroDNATrasnfectionReagent
-Cell-dependent3~4timeshigherefficiency
-Topchoiceforhard-to-transfectcells
-Efficiencyboostedinthepresenceofserumandantibioticsformostofcelltypes
-Exceptionalhighlevelsofrecombinantproteinproduction

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.

ComparisonsofTransfectionEfficiencyandCytotoxicityofGenJet(Ver.II)withOldVersionGenJetandOtherLeadingProducts

GenJet_Ver_II_vs_GenJet
Comparisonoftransfectionefficiency(leftpanel)andtoxicity(rightpanel)GenJetVer.IIwitholdversionGenJetoncommonlyusedmammaliancells.phRL-CMVencodingRenillaLuciferasewasintroducedintocellswiththepresenceofserumandantibiotics.TheRenilliaLuciferaseactivitywasdeterminedwithRenillaLuciferaaseAssaySystem(Promega).Forcytotoxicity,MTTassaywasemployedtodeterminecellviabilitywithuntransfectedcontrolas100%.

GenJet_Ver_II_vs_L2K
AcomparisonoftransfectionefficiencyofGenJet(Ver.II)vs.Lipofectamine2000onprimaryrataorticsmoothmusclecells.PrimaryrataorticsmoothmusclecellswerepreparedandtransfectedwithanucleartargetedGFPCDNAbyGenJet(rightpanel)andLipofectamine2000(L2K,leftpanel)respectively.ThetransfectionefficiencywerecheckedunderaNikonEclipse2000fluorescencemicroscopy48hoursposttransfection

GenJet_Ver_II_vs_GenJet_HEK293
GenJet_Ver_II_vs_GenJet_HELA
UpperPanel:TransfectionefficiencycomparisonofGenJetVer.IIwitholdversionGenJeton293Tcells.pEGFP-N3wastransfectedto293Tcellsat90%confluencywithpresenceofserumandantibiotics.GFPfluorescencewasdetectedwithNikonEclipse2000microscopy48hoursposttransfection
Lowerpanel:TransfectionefficiencycomparisonofGenJetVer.IIwitholdversionGenJetonHelacells.pEGFP-N3wastransfectedtoHelacellsat90%confluencywithpresenceofserumandantibiotics.GFPfluorescencewasdetectedwithNikonEclipse2000microscopy48hoursposttransfection

TechnicalInformation&Datasheet
-ACompleteTransfectionProtocol
-AShortProtocolforAdvancedUsers
-AProtocolforTransfectingSUSPension293andCHOCells
- AProtocolforLentivirusProduction
- AProtocolrAAVProduction
-TechnicalNote&TransfectionTips

RelatedProduct:GenJetInVitroDNATransfectionreagent

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品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。