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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/GenJet™ In Vitro DNA Transfection Reagent for Neuro-2A Cells/SL100489-N2A/1.0 mL
产品编号:SL100489-N2A-5x1.0mL
市  场 价:¥15760.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$788.00
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/GenJet™ In Vitro DNA Transfection Reagent for Neuro-2A Cells/SL100489-N2A/1.0 mL
商品介绍

Description

GenJet™DNAInVitroTransfectionReagentforNeuro2Aispre-optimizedfortransfectingNeuro2Acells.RefertothefollowingoptimaltransfectionconditionsformaximaltransfectionefficiencyonNeuro2Acells.GenJet™reagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor150to100transfectionsin6wellplates.

SummaryofOptimalTransfectionConditions:
Confluenceonthedayoftransfection
Cellcultureconditions
GenJet™(µl):DNA(µg)Ratio
DiluentforDNAandTransfectionReagent

IncubationTimetoFormGenJet™/DNAComplex
PresenceofSerum/AntibioticsduringTransfection
ChangeMedium5HoursAfterTransfection
MaximalEfficiency

TransfectionResults:
ReporterGene
Plasmid

Efficiency(GFP%)


~90%
DMEMwith4.5g/Lglucose,10%FBS

3:1
Serum-freeDMEMwith4.5g/Lglucose

15minutesatRT
Yes
Yes
48hours


EGFP
pEGFP-N3(CMVpromoter)

89%

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger


APictureShowingTransfectionEfficiencyofGenJet™ReagentonNeuro2ACells. Neuro2AcellsweregrownperATCCrecommendedculturemediumandtransfectedwithpEGFP-N3byGenJet™andvisualizedbyaNikonEclipseFluorescencemicroscopewithDICphase(leftpanel)andFITC(rightpanel)imaging36hoursposttransfection

DataSheet

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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