Signagen/GenJet In Vitro DNA Transfection Reagent for Neuro-2A Cells/SL100489-N2A/0.5 mL,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/GenJet In Vitro DNA Transfection Reagent for Neuro-2A Cells/SL100489-N2A/0.5 mL

产品编号：SL100489-N2A-5x1.0mL

市场价：¥15760.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$788.00

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

Description

GenJet DNA In Vitro Transfection Reagent for Neuro2A is pre-optimized for transfecting Neuro2A cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Neuro2A cells. GenJet reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium 5 Hours After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)

~90%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes
48 hours

EGFP
pEGFP-N3 (CMV promoter)
89%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

A Picture Showing Transfection Efficiency of GenJet Reagent on Neuro2A Cells. Neuro2A cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet and visualized by a Nikon Eclipse Fluorescence microscope with DIC phase (left panel) and FITC (right panel) imaging 36 hours post transfection

Data Sheet

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。