Signagen/GenJet In Vitro DNA Transfection Reagent for PC-12 Cell/SL100489-PC12/1.0 mL,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/GenJet In Vitro DNA Transfection Reagent for PC-12 Cell/SL100489-PC12/1.0 mL

产品编号：SL100489-PC12-5x1.0mL

市场价：¥15760.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$788.00

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

Description

GenJet DNA In Vitro Transfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla. PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor. This makes PC12 cells useful as a model system for neuronal differentiation.

Refer to the following optimal transfection conditions for maximal transfection efficiency on PC12 cells. GenJet reagent, 1.0 ml, is sufficient for 660 transfections in 24 well plates or 330 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)

~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours

EGFP
pEGFP-N3 (CMV promoter)
50%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。