Signagen/GenMute siRNA Transfection Reagent/SL100568/5 x 1.0 mL,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/GenMute siRNA Transfection Reagent/SL100568/5 x 1.0 mL

产品编号：SL100568-5x1.0mL

市场价：¥19760.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$988.00

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

GenMutesiRNATransfectionReagent

Description:
GenMuteReagentisanovelbiodegradablenon-LiposomalsiRNA/miRNA&DNAdeliverytool.WithourproprietarypHDependentConformationalChange(PDCC)technology,thebiodegradabletransfectionreagentwasformulatedbyadditionofpre-screenedhydrophobicgroupstoitssidechain,makingGenMuteReagentaversatileandmostpowerfulgenedeliverytoolinthemarket.GenMuteReagenthavebeenvalidatedtoeffectivelyandreproducIBLytransfectsinglesiRNA/miRNAorco-transfectDNA/siRNAorDNA/miRNAtovarietyofmammaliancells.

Size&content:
-GenMuteReagent,1.0mL,sufficientfor~2,000reactionsbasedontransfecting5.0pmolsiRNAormiRNAmimicsin24-wellplate.
-GenMuteTransfectionBuffer(5x),formulatedformaximaltransfectionefficiency,8.0mLtomake40mLworkingsolution.

Applications:
-SinglesiRNAtransfectionorDNA/siRNAco-transfectiontovarietyofmammaliancells.
-SinglemiRNA,miRNAmimicsorinhibitorstransfectionorDNA/miRNAorDNA/miRNAmimicsco-transfectiontovarietyofmammaliancells.

Storage:
Storeat4°CforGenMuteReagentandRTforGenMuteTransfectionBuffer(5x). Ifstoredproperly,theproductisstablefor12monthsorlonger.

Advantages:
-Excellentsilencingatlowconcentrationswithonly1.0nMsiRNA/miRNAmimics
-Minimizingnon-specificandofftargeteffectsinthecell
-Onetubereaction
-Bestforbroadmammaliancells
-Verylowcytotoxicity
-Veryaffordable

ComparisonsofSilencingEfficacyofGenMutesiRNA&DNATransfectionReagentwithBrandNameProducts

KnockdownefficacycomparisonofGenMuteTransfectionReagent(upperpanel)vs.Dharmafect4siRNATransfectionReagent(middlepanel)andLipofectamine2000(lowerpanel)onHEK293cells.siRNAtargetingrenillaluciferaseatdifferentfinalconcentrationsrangingfrom0.5to20nMwasco-transfectedwithrenillaluciferasegene(0.5µgofpRL-CMVDNAperwell)bytheabovethreetransfectionreagentspermanufacturers"protocolsintoHEK293cellsgrowingona24-wellplate.Renillaluciferaseactivitywasdetermined24hafterpostco-transfectionwithrenillaluciferasedeterminationsystem(Promega).Theluminescencewasmeasuredfrom5.0µloflysateduring10sintegrationwithaluminometer(BeckmanCoulterLD400).Luciferaseactivitywasexpressedaslightunitsintegratedover10s(RLU)andnormalizedpermgofcellproteinbyusingtheBCAassay.Theerrorsbarsrepresentstandarddeviationderivedfromtriplicateexperiments.Luciferase-silencingefficiencywascalculatedrelativetountreatedcells.WhileGenMuteandDharmafect4reagentsdeliveredsignificantgenesilencingfrom1.0nMofrenillaluciferasesiRNA,lipofectamine2000gavegoodknockdownonlyafter30nM(datanotshown).

ExcellentsilencingofendogenouslyexpressedKIF11(alsoknownasEg5)inHEK293cellswith0.5µlofGenMutereagentand5.0pmolEg5siRNAperwellof24-wellplate.KIF11(alsoknownasEg5)encodesamotorproteinthatbelongstothekinesin-likeproteinfamilyinvolvedinchromosomepositioningandbipolarspindleformationduringcellmitosis.AreductioninKIF11levelscausesmitoticarrest.GenMutereagenteffectivelydeliversEg5siRNA(final10nM,rightpanel)toHEK293cellsatonly0.5µlperwellof24-wellplatevs.negativecontrol(final10nM.leftpanel).Comparedwithnegativecontrol(leftpanel),phenotypeof"rounded-up"293cellswerevisualized24hoursposttransfection(rightpanel)withaNikonmicroscope.

GenMuteTransfectionReagentknockeddownendogenouslaminA/CgeneexpressioninHelacells.AsiRNAtargetinglaminA/Cgene(rightpanel)andashamsiRNA(leftpanel)wereintroducedintoHelacells(final1.0nM)byGenMuteTransfectionReagent.LaminA/Cgenesilencingwasmonitored24hposttransfectionbyimmunofluorescence.LaminA/CwasprobedwithamousemonoclonallaminA/CspecificantibodyfollowedbyadditionofFITC-conjugatedanti-mouseantibodies.QuantitativeanalysisshowedthatlaminsiRNAat1.0nMdeliveredbyGenMuteTransfectionReagentknockeddown94%endogenouslyexpressedlaminA/CinHelacells.

DataSheet&Protocol
-AProtocolforsiRNATransfectionandDNA/siRNACo-Tranafection
-AProtocolformiRNAMimicsTransfectionandDNA/miRNACo-Transfection
-AProtocolforDNATransfectiontoMammalianCells

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品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。