Description:
HapiCellTransfectionToxicityRemovalCocktailwasourproprietaryformulationforeffectivelyeliminatingDNAorsiRNAtransfectionmediatedtoxicity. HapiCellTransfectionToxicityRemovalCocktailwasdevelopedtocontainamixtureofseveralchemicalswhichwereconfirmedtoeffectivelyremoveDNA/siRNAtransfectionmediatedcytotoxicity. Incubationofsensitivecells(e.g.,SaoS-2,HUVEC,primarykerotinocytes,etc)withHapiCellToxicityRemovalReagentfor2minutesatRTwasconfirmtocompletelyremovethetransfectionrelatedtoxicitywhichotherwisekillthecells. Thecocktailwasusedforchemicaltransfectiononly,notforelectroporation. HapiCellTransfectionToxicityRemovalCocktail,125ml,issufficienttotreat125wellsfor24-wellplateand63wellsfor6-wellplaterespectively.
Feature:
-Removestransfectionmediatedtoxicityafter2minutesincubationatRT
-Nontoxictocells
-MaintaincellviABIlity
-Improvetransfectionefficiency
-Easytouse
HapiCellTransfectionToxicityRemovalCocktailEffectivelyRemoveTransfectionMediatedToxocity:
AexampleshowingcompleteremovaloftransfectiontoxicityaftertreatmentwithHapiCellTransfectionToxicityRemovalCocktailonmouseskinfibroblast.ADNAencodingGFPwasdeliveredtomouseskinfibroblastsbyLipofectamine2000inabsenceofserum.Serum-containingmediumwaschangedback5hoursposttransfectionfollowed2minutesincubationwith(rightpanel)orwithout(leftpanel)HapiCellTransfectionToxicityRemovalCocktail.Thecellviabilitywascheckedundermicroscope24hoursposttransfection
AexampleshowingcompleteremovaloftransfectiontoxicityaftertreatmentwithHapiCellTransfectionToxicityRemovalCocktailonprimarymousekerotinocytes.ADNAencodingGFPwasdeliveredtoprimarymousekerotinocytesbyLipofectamine2000inabsenceofserum.Serum-containingmediumwaschangedback5hoursposttransfectionfollowed2minutesincubationwith(rightpanel)orwithout(leftpanel)HapiCellTransfectionToxicityRemovalCocktail.Thecellviabilitywascheckedundermicroscope24hoursposttransfection
DataSheet
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606),SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。