Description:
Basedonourinnovativeandproprietarylipid-conjugationtechnology,LipoJet™TransfectionKit,formulatedfromnovelfluorinatedcationiclipids,exhibitssignificantdifferencefromotherlipidstransfectionreagentsinthemarket.LipoJet™TransfectionKitisthemostpowerfulyetverygentlegenedeliverytoolforavarietyofapplicationsincludingplasmidDNAand/or siRNAformostofmammaliancelltypes. ComparedwithleADIngproductsinthemarket, LipoJet™ismore cost-effectiveandalways provideshighertransfectionefficiencywithlesscytotoxicity.
Kitcontent:
-LipoJet™Reagent,1.0mlsufficientfor1000DNAtransfectionsand1000siRNAtransfections (24-wellplate).
-LipoJet™TransfectionBuffer(5x),8.0mLtomake40mLofworkingsolution(1x).
Features:
-Versatile:DNAtransfection,siRNAtransfectionandDNA/siRNAco-transfection.
-Powerful: Outstandingefficiencyonadherentcells.
-Extremelylowtoxicity: ImproveCellviABIlityaftertransfection
-Economical:Moretransfectionswithlessreagent
-Easytouse:OnetubereactionandcompatIBLewithserum/antibiotics.
-Suitableforhigh-throughputapplication
Storage:
Storeat4°CforLipoJetReagentandRTforLipoJetTransfectionBuffer(5x). Ifstoredproperly,theproductisstablefor12monthsorlonger.
BroadTransfectionSpectrumforMammalianCellTypes
CellLine | DNATransfection | siRNATransfection |
293,293T | 85% | - |
ExamplesShowingExcellentDNAandsiRNATransfectionEfficiencyofLipoJet™InVitroTransfectionKit
AefficiencycomparisonofLipoJet™reagentvs.brandnameproductstotransfectHela,Cos-7,NIH-3T3,CHOandHEK293(leftpanel)andCos-7(rightpanel). pEGFP-N3CDNA(0.5µg/wellof24-wellplate)wastransfectedintodifferentmammaliancellsperthestandardtransfectionprotocolsinpresenceofserum(10%FBS)andantibioticsasrecommendedbymanufacturers. ThetransfectionefficiencywereanalyzedviaFACS48hoursposttransfection.
AtoxicitycomparisonofLipoJet™reagentvs.brandnameproductstotransfectHelacells. pEGFP-N3cDNA(1.0µg/wellof24-wellplate)wastransfectedtoHelacellsperthestandardtransfectionprotocolsinpresenceofserum(10%FBS)andantibioticsasrecommendedbymanufacturers. TheMTTassay(rightpanel)andphasecontrastimaging(leftpanel)wereusedtoanalyzethecellviability48hoursposttransfection.
AcomparisonofLipoJet™reagentvs.Lipofectamine2000(L2K)andPolyJet™transfectionreagentonHEK293Tcell.pEGFP-N3cDNA(0.125µg/well,0.25µg/welland0.5µg/perwellof24-wellplate)wastransfectedinto293Tcellsusingthestandardtransfectionprotocolsinpresenceofserum(10%FBS)withLipoJet™(upperpanelatLipoJet™/DNA(µl/µg)ratio=2),L2K(middlepanelatL2K/DNA(µl/µg)ratio=3)andPolyJet™(lowerpanelatatPolyJet™/DNA(µl/µg)ratio=3)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope48hoursposttransfection.
AcomparisonofLipoJet™reagentvs.Lipofectamine2000(L2K)andPolyJet™transfectionreagentonHelacell. pEGFP-N3cDNA(0.125µg/well,0.25µg/welland0.5µg/perwellof24-wellplate)wastransfectedintoHelacellsusingthestandardtransfectionprotocolsinpresenceofserum(10%FBS)withLipoJet™(upperpanelatLipoJet™/DNA(µl/µg)ratio=2),L2K(middlepanelatL2K/DNA(µl/µg)ratio=3)andPolyJet™(lowerpanelatatPolyJet™/DNA(µl/µg)ratio=3)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope48hoursposttransfection.
ExceptionalgenesilencingofLipoJet™reagentduringDNA/siRNAco-transfectiononmammaliancells.TransfectionofGFPcDNA(leftpanel,0.25µgperwellof24-wellplate)andco-transfectionofGFPcDNA(0.25µgperwellof24-wellplate)andGFPtargetedsiRNA(final10nM,rightpanel)withLipoJet™reagentleadstoexceptionalgenesilencingonHEK293(upperpanel),Hela(middlepanel)andSaoS-2(lowerpanel)cellsrespectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope48hoursposttransfection.
ExceptionalDNA/siRNAco-transfectionefficiencyonHUVEC.Co-transfectionofmCherrycDNA(0.10µgperwellof24-wellplate)andFITCconjugatedsiRNA(final30nMperwellof24-wellplate)toHUVECwithLipoJet™reagentgaveriseto60%mCherry+(phasecontrastoverlappedwithmCherryimaging,leftpanel)andnearly100%FITC-siRNA+(phasecontrastoverlappedwithFITCimaging,rightpanel)HUVEC24hoursaftertransfection. ThepicturesweregivenfromDr.PanKongofUSCascourtesy.
ExcellentsilencingofendogenouslyexpressedKIF11(alsoknownasEG5)inHEK293(upperpanel)andHela(lowerpanel)cellswithLipoJet™reagentat10nMEG5siRNA.KIF11(alsoknownasEG5)encodesamotorproteinthatbelongstothekinesin-likeproteinfamilyinvolvedinchromosomepositioningandbipolarspindleformationduringcellmitosis.AreductioninKIF11levelscausesmitoticarrest.LipoJet™reagenteffectivelydeliversEG5siRNA(final10nM)toHEK293andHelacells,leadingtomorethan80%of"round-up"phenotypeofHEK293andHelacells24hposttransfectionovernegativecontrol(final10nMwithshamEG5siRNA).Thephenotypeof"rounded-up"HEK293andHelacellswerevisualized24hposttransfectionwithaNikonmicroscope.
AimageshowingexceptionaltransfectionefficiencyofLipoJetTransfectionKitonHumanembryonicstemcells (hESCs). ThehESCsgrowninE8mediumonGeltrexvs(leftpanel,DICimaging)wastransfectedwithpEF1α-GFP.TheGFPexpression(rightpanel)wasvisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection.TheabovepictureswereprovidedbyDr.MarinaPryzhkovaofJohnsHopkinsUniversityascourtesy
DataSheet&Protocol:
-AProtocolforDNAandsiRNATransfection
-AShortProtocolforDNATransfection
-AShortProtocolforsiRNATransfection
-AProtocolforDNA/siRNACo-transfection
- TechnicalNote&TransfectionTips
Weareoffering"WirelessPowerPointPresenter"tocustomerswhopurchase5x1.0mLLipoJet™reagentbyDecember2014.Takeadvantageofitanddon"tletthischancego...
FG-3:WirelessPowerPointPresenterwithCaseforpurchaseof5X1.0mlormore.Thispresenterisofferedforlimitedtimeonly! |
Torequestafreetrialsample,pleaseCreateAnAccountwithustoenteryourshippingaddressandemailusatorder@Signagen.com
Testimonials:
Sorryforsooobigdelay,butnowIamtotallyinlovewithbothPolyJetandLipoJet.RecentlywediscoveredthatLipoJethassupergoodtransfectionefficiencywithmESCsandhESCswithi muchlesstoxicity.LastweekItransfectedovernighthumanESClineH1usingLipoJetwithsameamazinglyhighefficiency-upto50~70%.DNAconstructincludesGFPunderEF1apromoter(CMVpromoterdoesnotworkinhESCs).ThisisthebesttransfectionefficiencyIeversawwithESCs.Lipofectamin3000didn"tgivemetheseresults(usedonmouseESCs).
--------MarinaPryzhkova,Ph.D.,JHU
Itriedtheplasmid/siRNAco-transfectionusingtheLipoJetsampleinEAhy926cellwhichisaHUVECcellline.Theresultsareprettygood,atleastforthetransfectionefficiency.Fortheplasmidtransfection,48hourslater,around60%cellaretransduced.ForsiRNA,theefficiencyisalmost100%.WeareorderingmoreLipoJetreagenttousefromnow.
-----Dr.PanKong,UniversityofSouthernCalifornia
Wegot>90%efficiencywithLipoJetvs.80%withX-tremeGENE9on293Tcell.Definitelywillordermore....
-----Dr.NuoYangfromRoswellParkCancerInstitute
HerearetheresultsfromourpreliminaryexperimentswithLipoJet.Weareverysatisfiedwithitsefficiency.Unfortunately,wewereoutofFugene6/HDandwereunabletotestthemsidebyside,butbasedonpreviousexperience,wedobelievethatyourproductworkedwithbetterefficiency.
------Dr.AlisonMcKelveyfromUniversityofPittsburgh
IamhappytosaythatIhadgreatsuccesswithyourtransfectionreagentsonOKF6/TERT2humankeratinocytes.IwillputtogetherthedataonceIhavecompletedmyanalysis.IreallyliketheLipoJet.MycellofinterestdidnotdoverywellintheGenMutealthoughmycontrolcellsdid.WewanttogoaheadwithorderingASAP.Ialsohaveacouponfor10%foraPOorder.
-----Dr.MichelleSimpson-AbelsonfromUPMC
WedidindeedtestthemsidebysidewithourhomemadeCaPhos.Theresultsaregreat, LipoJetandCalFectinbothdidextremelywell.LipoJetbeingthebetterofthetwo.Iwillsendyoutheresultswhen Igetthem, Iamcurrentlyoutofthelabbutwewillbeorderingmoreforcertain.Thankyouforsendingthosesamples.
------Dr.AhmedHassib,CornellUniversity
FortheLipoJet,IonlycompareditwithLipoLTXinHelacells.TheLipoJetgaveamuchhigherefficiency(~35%betterthanLipoLTX)24hoursaftertransfection.WewillordersomeLipoJet.
-----AbetatesterfromUSC
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606),SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。