Application:SubstrateofHorserADIshperoxidaseforchemiluminescentdetectionofproteininWesternBlot

Features:
▪Chemiluminescencelastsforupto6hourswithoutsignificantattenuation
▪Extremelysensitiveabletodetect0.1pgproteinwithverylowbackground
▪Extremelystableforstorageupto12mouthsat4°C
▪Workingsolutionstableupto72hoursat4°C

ComparisonsofLumiGOLD™ECLWesternBlottingDetectionKitwithOtherLeadingBrandProducts:
A.LumiGOLD™ECLWesternBlottingDetectionofEGFP(enhancedgreenfluorescentprotein)inWideLinearRange*




*10two-folderserialdilutionsofpurifiedEGFP(0.1pg~51.2pginlane1~10fromlefttoright,upperpanel).EGFPwasseparatedbySDS-PAGEelectrophoresisfollowedbytransfertoaPVDFmembrane.Themembranewasblockedwith5%skimmilk(product#SL100317)andwashedwithTBSTfollowedbyincubationofmouseanti-EGFPmonoclonalantibody.HRP-conjugatedgoatanti-mouseIgGwasusedtorecognizethemouseanti-GFP.TheblotwasexposedtoFujifilmfor20seconds.ThelinearrangeofLumiGOLDD™ECLkitwasdetermined(lowerpanel)byadditionof10two-folderserialdilutedhorseradishperoxidase(HRP)totheLumiGOLD™workingsolutionfollowedbydeterminationofluminositybyBeckmanCoulterLD400luminometer

B.AcomparisonofLumiGOLD™ECLWesternBlottingDetectionKitwithPopularBrandProducts

Products	Noise*	Signal**	Signal/Noise
LumiGOLD™	18905	1938181	103
AmershamECL™	1495	93098	62
AmershamECL™Plus	320478	3480213	10.9
PiercePicoFemto™	362103	3353736	9.3 *Noisereferstothebackgroundchemiluminescenceoftheworkingsolutionwithoutadditionofhorseradishperoxidase(HRP) **SignalreferstotheHRPcatalyzedchemiluminescenceoftheworkingsolutionafteradditionof5µlxHRP-conjugatedgoatanti-mouseIgG(100,000dilution).LuminositywasdeterminedbyaBeckmanCoulterLD400luminometer C.LumiGOLD™ECLWesternBlottingDetectionofbeta-ActininComparisonofAmershamECLKit*           AmershamECL™                  LumiGOLD™ *HEK293celllysatewaspreparedwithCompLysis™ProteinExtractionReagent(Cat#SL100319)andthesameamountoftotalproteinswereloadedtothecorrespondingwells(lane#1=lane#5/lane#2=lane#6/lane#3=lane#7/lane#4=lane#8).beta-Actinwaspickedupbyanti-actinantibodyfollowedbydevelopmentwithECLreagentsfor2minutes.Theexposuretimewaslabeledintheaboveimages DataSheet Testimonials Ihaveuseditforseveraltimes,itisgoodaccordingtotheresults.Itseemsthatthisproductisbetterthanotherstosomedegree... ------byDr.AibingWang,NHLBI,NIH 品牌介绍 SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。 公司介绍 更多... SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。 产品分类 更多... G-protein Coupled Receptors Transcription Factors Adenovirus Production Service Lentivirus Production Service Ion Channels Pre-made AAVs Free Offers Endopeptidases & Exopeptidases Membrane Receptors Protein Kianses Miscellaneous Ready to Package Adenoviruses AAV Production Service Cytokines Tumor and Apoptosis In Vivo Transfection Reagents Clones for Signal Transduction Protein Kinase, Dead Mutants or Dominant Negatives Phosphoric Diester Hydrolases In Vitro siRNA Transfection Reagents Western Blotting Products Transfection Accessories Phosphoprotein Phosphatases shRNA/miRNA Adenovirus Svc shRNA/miRNA AAV Service Pre-optimized Transfection Reagents In Vitro DNA Transfection Reagents Pre-made Adenovirus Pre-made Lentivirus Ligand-dependent Nuclear Receptors Cytochrome P450 销售排行 更多... Signagen/C21orf127 Adenovirus/Category: Ready to Package Adenovirus/ $618.00 Signagen/CYS1 Adenovirus/Category: Ready to Package Adenovirus/ $618.00 Signagen/ZSCAN23 Adenovirus/Category: Ready to Package Adenovirus/ $618.00 Signagen/ZSWIM7 Adenovirus/Category: Ready to Package Adenovirus/ $618.00 Signagen/NYD-SP26 Adenovirus/Category: Ready to Package Adenovirus/ $618.00 Signagen/HAVCR2 Adenovirus/Category: Ready to Package Adenovirus/ $618.00 Signagen/ZRANB3 Adenovirus/Category: Ready to Package Adenovirus/ $618.00 Signagen/ZXDB Adenovirus/Category: Ready to Package Adenovirus/ $618.00 问答列表 更多... GenFectinTM基因转染试剂 0.5ml_品牌馆_医流商城分销服务和交易平台 Array 厂家直采 全球直采 正品优价 正品保障 厂家直发 有线跟踪 正规清关 CIF100%正规报关，提供发票 及时交付 限时必达 不达必赔 快速导航： 公司介绍 产品中心 新闻动态 在线咨询 联系我们 公司介绍 产品中心 新闻动态 在线咨询 联系我们 总部地址： 江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125) 工作时间：周一至周五9:00~18:00 技术支持： Email：info@ebiomall.com 短信：18915418616 服务热线： 4000-520-616（全国） ☏  0512-67156496（苏州） 版权信息： ©  2015- 苏州蚂蚁淘生物科技有限公司 版权所有 中国 网站备案： 苏ICP备17049038号-1 苏公网安备 32059002001627号 友情链接： 联系QQ:764052152 signalchem现货 spherotech试剂抗体 sterck代理商 usbio促销 usascientific公司 trc supertechs sysmexpartec terrace tebubiobps 免责声明： 本站有部分内容来自互联网，如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权，请来电或致函告之， 本网站将在规定时间内给予删除等相关处理。 扫描二维码关注官方微信 获取最新促销及产品信息