Application:SubstrateofHorserADIshperoxidaseforchemiluminescentdetectionofproteininWesternBlot
Features:
▪Chemiluminescencelastsforupto6hourswithoutsignificantattenuation
▪Extremelysensitiveabletodetect0.1pgproteinwithverylowbackground
▪Extremelystableforstorageupto12mouthsat4°C
▪Workingsolutionstableupto72hoursat4°C
ComparisonsofLumiGOLD™ECLWesternBlottingDetectionKitwithOtherLeadingBrandProducts:
A.LumiGOLD™ECLWesternBlottingDetectionofEGFP(enhancedgreenfluorescentprotein)inWideLinearRange*
*10two-folderserialdilutionsofpurifiedEGFP(0.1pg~51.2pginlane1~10fromlefttoright,upperpanel).EGFPwasseparatedbySDS-PAGEelectrophoresisfollowedbytransfertoaPVDFmembrane.Themembranewasblockedwith5%skimmilk(product#SL100317)andwashedwithTBSTfollowedbyincubationofmouseanti-EGFPmonoclonalantibody.HRP-conjugatedgoatanti-mouseIgGwasusedtorecognizethemouseanti-GFP.TheblotwasexposedtoFujifilmfor20seconds.ThelinearrangeofLumiGOLDD™ECLkitwasdetermined(lowerpanel)byadditionof10two-folderserialdilutedhorseradishperoxidase(HRP)totheLumiGOLD™workingsolutionfollowedbydeterminationofluminositybyBeckmanCoulterLD400luminometer
B.AcomparisonofLumiGOLD™ECLWesternBlottingDetectionKitwithPopularBrandProducts
Products | Noise* | Signal** | Signal/Noise |
LumiGOLD™ | 18905 | 1938181 | 103 |
AmershamECL™ | 1495 | 93098 | 62 |
AmershamECL™Plus | 320478 | 3480213 | 10.9 |
PiercePicoFemto™ | 362103 | 3353736 | 9.3 *Noisereferstothebackgroundchemiluminescenceoftheworkingsolutionwithoutadditionofhorseradishperoxidase(HRP) 品牌介绍
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606),SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。
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