ProductCategory:Pre-maderecombinantlentivirus;LV-Synapsin-tdTOMATO;LV-Syn-tdTOMATO;Synapsin-tdTOMATOLentivirus
Description:LV-Synapsin-tdTOMATO isapre-madelentiviruswhichexpressestdTOMATOundertheSynapsinpromoterwhcihdirectstdTOMATOexpressionexclusivelyinneurons.tdTOMATO,tandemdimeric(pseudo-monomeric)derivativeofDsRed, isanexceptionallybrightredfluorescentproteinwitharound6timesbrighterthanEGFP. BecausethetwosubunitsoftdTomatoarelinkedcovalently,itbehavesasamonomer,andhasbeenusedsuccessfullyforN-andC-terminalfusions.Itshowsverylowaggregation,excellentphotostABIlityanditshalf-timeformaturationisjustonehourat37°C.TheredemissionandbrightnessoftdTomato’smakeitidealforbothinvitroandinvivoimagingstudies.
Titer:>1x109TU/mL
SelectionAntibiotic:No
Purity*: InvivogradeandOKforbothinvitrotissuecultureinfectionandinvivoinjection
Size: 2x25 µL(StdPack)
StorageBuffer:PBS
InfectionProtocol:
TransferVectorMap:
*Concentratedvia PEGprecipitationfollowedbyultra-centrifugationat25000rpm
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