Product Category: Pre-made recombinant lentivirus; LV-EF1α-iCre-Puro; LV-EF1A-iCre-Puro; LV-EF1α-iCre-IRES-Puro; EF1A-iCre-Puro Lentivirus; EF1α-iCre-Puro Lentivirus

Description: Cre recombinase is a type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites.  Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.  By optimizing the mammalian codon usage for Cre recombinase, Cre expression is improved in the mammalian cells. This codon-improved Cre (known as iCre) transgene shows lower CpG content from the prokaryotic coding sequence, thus reducing the chances of epigenetic silencing in mammals.

This LV-EF1α-iCre-Puro pre-made lentivirus expresses iCre recombinase with co-expression of puromycin selection in an IRES cassette under EF1α promoter. Ready to use format.

Titer: > 1x10⁹ TU/mL

Selection Antibiotic: Puromycin

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol:

Transfer Vector Map:


* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm