ProductCategory:Pre-maderecombinantlentivirus;LV-EF1α-iCre-Puro;LV-EF1A-iCre-Puro;LV-EF1α-iCre-IRES-Puro;EF1A-iCre-PuroLentivirus;EF1α-iCre-PuroLentivirus
Description: CrerecombinaseisatypeItopoisomerasefrombacteriophageP1thatcatalyzesthesite-specificrecombinationofDNAbetweenloxPsites. Crerecombinaseisusedasatooltogeneticallymodifygenes,suchastodeleteasegmentofDNAflankedbyLoxPsitesincellsorexperimentalanimals. ByoptimizingthemammaliancodonusageforCrerecombinase,Creexpressionisimprovedinthemammaliancells.Thiscodon-improvedCre(knownasiCre)transgeneshowslowerCpGcontentfromtheprokaryoticcodingsequence,thusreducingthechancesofepigeneticsilencinginmammals.
ThisLV-EF1α-iCre-Puropre-made lentivirus expressesiCrerecombinasewithco-expressionofpuromycinselectioninanIREScassetteunderEF1αpromoter.Readytouseformat.
Titer:>1x109TU/mL
SelectionAntibiotic:Puromycin
Purity*: InvivogradeandOKforbothinvitrotissuecultureinfectionandinvivoinjection
Size: 2x25 µL(StdPack)
StorageBuffer:PBS
InfectionProtocol:
TransferVectorMap:
*Concentratedvia PEGprecipitationfollowedbyultra-centrifugationat25000rpm
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