Signagen/PepMute Plus siRNA Transfection Reagent/SL100571/5 x 1.0 mL,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/PepMute Plus siRNA Transfection Reagent/SL100571/5 x 1.0 mL

产品编号：SL100571-5x1.0mL

市场价：¥34960.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$1748.00

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

PepMutePlussiRNATransfectionReagent

Description:
PepMutePlussiRNA&DNATransfectionReagentisanupgradedversionofPepMutereagent(Cat#SL100566)bycrosslinkingpre-screenedhydrophobicaminoacidstoitsbackbone.WithourproprietarypHDependentConformationalChange(PDCC)technology,thetransfectionreagentwhichcontains36aminoacidslong"speptidebackbonewasformulatedbyadditionofpre-screenedhydrophobicaminoacidstoallaminegroups,conferringPepMutePlusreagentanABIlitytoself-assemblyduringformationoftransfectioncomplexandmakingPepMutereagentaversatileandmostpowerfulgenedeliverytoolwhichprovidesmorethan95%silencingefficiencyat1nMsiRNAinvarietyofmammaliancells.PepMutePlusReagenthavebeenvalidatedtoeffectivelyandreproducIBLytransfectsingleDNAorsiRNAorco-transfectDNA/siRNAtovarietyofmammaliancells.

Figure1.AcartoonshowingPepMutePlussiRNATransfectionReagentwasdeveloped

Size&content:
-PepMutePlusTransfectionReagent,1.0mL,sufficientfor~1000reactionsbasedontransfecting2.5pmolssiRNAin24-wellplate
-PepMutePlusTransfectionBuffer(5x),formulatedformaximaltransfectionefficiency,8.0mL

Storage:
Storeat4°CforPepMutePlusReagentandRTforPepMuteTransfectionBuffer(5x). Ifstoredproperly,theproductisstablefor12monthsorlonger.

Advantages:
-Effectiveknockdown(95%)withonly0.5pmolsiRNA(1.0nMsiRNAin24-wellplate)
-Formulatedforhard-to-transfectmammaliancells
-Onetubereactionandeasytransfectionprotocol
-Bestforbroadspectrumespeciallyhard-to-transfectmammaliancells
-Verylowcytotoxicity

ComparisonsofKnockdownEfficacyofPepMutePlussiRNA&DNATransfectionReagentwithBrandNameProducts

Figure2.KnockdownefficacycomparisonofPepMutePlusTransfectionReagent(upperpanel)vs.Dharmafect4siRNATransfectionReagent(middlepanel)andLipofectamine2000(lowerpanel)onHEK293cells.siRNAtargetingrenillaluciferaseatdifferentfinalconcentrationsrangingfrom0.5to20nMwasco-transfectedwithrenillaluciferasegene(0.5µgofpRL-CMVDNAperwell)bytheabovethreetransfectionreagentspermanufacturers"protocolsintoHEK293cellsgrowingona24-wellplate.Renillaluciferaseactivitywasdetermined24hafterpostco-transfectionwithrenillaluciferasedeterminationsystem(Promega).Theluminescencewasmeasuredfrom5.0µloflysateduring10sintegrationwithaluminometer(BeckmanCoulterLD400).Luciferaseactivitywasexpressedaslightunitsintegratedover10s(RLU)andnormalizedpermgofcellproteinbyusingtheBCAassay.Theerrorsbarsrepresentstandarddeviationderivedfromtriplicateexperiments.Luciferase-silencingefficiencywascalculatedrelativetountreatedcells.WhilePepMutePlusandDharmafect4reagentsdeliveredsignificantgenesilencingfrom1.0nMofrenillaluciferasesiRNA,lipofectamine2000gavegoodknockdownonlyafter30nM(datanotshown).

Figure3.PepMutePlusTransfectionReagentknockeddownGFPgeneexpressioninHepG2cells.GFPCDNA,pEGFP-N3,wasco-transfectwithasiRNAtargetingGFPgene(final5.0nM,rightpanel)andashamsiRNA(final5.0nM,leftpanel)intoHepG2cellsbyPepMutePlusTransfectionReagent.GFPfluorescencewasvisualized24hposttransfectionwithaNikonT2000fluorescencemicorscopy.QuantitativeanalysisshowedthatGFPsiRNAat5.0nMdeliveredbyPepMutePlusTransfectionReagentknockeddown96%co-transfectedGFPexpressioninHepG2cells.

DataSheet&Protocol
-AProtocolforDNA/siRNACo-TranafectiontoMammalianCells
-AProtocolforsiRNATransfectiontoMammalianCells
-AProtocolforDNATransfectiontoMammalianCells

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品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。