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主营:DNA和siRNA转染试剂,病毒基因载体
℡ 4000-520-616
℡ 4000-520-616
Signagen/PepMute™ siRNA Transfection Reagent/SL100566/5 x 1.0 mL
产品编号:SL100566-5x1.0mL
市  场 价:¥19760.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$988.00
品      牌: Signagen
公      司:SignaGen Laboratories
公司分类:
Signagen/PepMute™ siRNA Transfection Reagent/SL100566/5 x 1.0 mL
商品介绍
PepMute™siRNATransfectionReagent

Description:
PepMute™siRNATransfectionReagent,formulatedfromsimulationofviruscellpenetratingpeptides(CPPs),isatotalnovelsiRNAdeliverytoolwhichprovidesmorethan95%silencingefficiencyat1nMsiRNAinvarietyofmammaliancells.Withourproprietarypeptidesimulationtechnology(PST),hundredsofviralCPPsweresimulated,synthesizedandscreenedforgenedeliveryefficacyinvarietyofmammaliancells(Figure1).PepMute™reagentwasthenidentifiedandvalidatedasanexceptionallyefficientvectorforcondensingandtransfectingshort(under100bp)singleordoublestrandednucleicacidssuchassiRNA,miRNAmimicsandDNAoligoestowidespectrumofmammaliancells.

How_PepMute_Wroks
Figure1.AcartoonshowingPepMute™siRNATransfectionReagentwasdevelopedbyPST

Size:
-PepMute™Reagent,1.0mL,sufficientfor~1,333reactionsbasedontransfecting0.5~5.0pmolsiRNAormiRNAmimicsin24-wellplate
-Pep
Mute™TransfectionBuffer(5x),formulatedformaximaltransfectionefficiency,8.0mLat5xconcentratedstocksolutiontomake40mLofworkingsolution

Applications:
-siRNAmiRNAmimicsormRNAtransfection
-DNA/siRNAco-transfection
-DNAoligoestransfection

Storage:

Storeat4°CforPepMute™ReagentandRTforPepMute™TransfectionBuffer(5x). Ifstoredproperly,theproductisstablefor12monthsorlonger.

Advantages:
-Excellentsilencingatfinal1.0nMsiRNA
-Over95%genesilencinginawidevarietyofcells
-Onetubereactionwitheasystandardprotocol
-CompatIBLewithserumandantibiotics
-ProtocolsadaptedtoHTS
-Verylowcytotoxicity
-Veryaffordable

SilencingEfficacyComparisonbetweenPepMute™siRNATransfectionReagentandLeADIngProducts
PepMute_vs_INTERFERIN_Dharmafect_jetPRIME
Figure2.ExcellentsilencingofendogenouslyexpressedKIF11(alsoknownasEG5)inHEK293cellswith1.0µlofPepMu
te™reagentand0.5pmolEG5siRNAperwellof24-wellplate.KIF11(alsoknownasEG5)encodesamotorproteinthatbelongstothekinesin-likeproteinfamilyinvolvedinchromosomepositioningandbipolarspindleformationduringcellmitosis.AreductioninKIF11levelscausesmitoticarrest.PepMute™reagenteffectivelydeliversEG5siRNA(final1.0nM)toHEK293cells,leadingtomorethan80%of"round-up"phenotypeofHEK293cells48hposttransfectionovernegativecontrol(final1.0nMwithshamEG5siRNA)whileleadingsiRNAtransfectinreagents,Lipofectamine™RNAiMAX(RNAiMAX,1.0nMEG5siRNA)/INTERFERin(1.0nMEG5siRNA)/Dharmafect(10.0nMEG5siRNA)andjetPRIME(20nMEG5siRNA)giveaverage37%,23%,53%and48%ball-shapedphenotyperespectivelyonHEK293cells.Thephenotypeof"rounded-up"293cellswerevisualized(upperpanel)andquantified(lowerpanel)48hposttransfectionwithaNikonmicroscope.

PepMute_vs_RNAiMAX_Dharmafect
Figure3.SilencingefficiencycomparisonofPepMute™TransfectionReagent(upperpanel)withDharmafect4(middlepanel)andLipofectamin
e™RNAiMAX(RNAiMAX,lowerpanel)siRNATransfectionReagentsonA549cells.
siRNAtargetingrenillaluciferaseatdifferentfinalconcentrationsrangingfrom0.5to20nMwasco-transfectedwithrenillaluciferasegene(0.5µgofpRL-CMVDNAperwell)bytheabovethreetransfectionreagentspermanufacturers"protocolsintoA549cellsgrowingona24-wellplate.Renillaluciferaseactivitywasdetermined36hafterpostco-transfectionwithrenillaluciferasedeterminationsystem(Promega).Theluminescencewasmeasuredfrom5.0µloflysateduring10sintegrationwithaluminometer(BeckmanCoulterLD400).Luciferaseactivitywasexpressedaslightunitsintegratedover10s(RLU)andnormalizedpermgofcellproteinbyusingtheBCAassay.Theerrorsbarsrepresentstandarddeviationderivedfromtriplicateexperiments.Luciferase-silencingefficiencywascalculatedrelativetountreatedcells.WhilePepMute™andDharmafect™4reagentsdeliveredsignificantgenesilencingfrom1.0nMofrenillaluciferasesiRNA,Lipofectamine™RNAiMAXgavegoodknockdownonlyafter20nMwhileenhancedgeneexpressionatlowconcentrationofsiRNA(0.5and1.0nMrespectively)wasobserved.

PepMute_GFP_siRNA_MCF7
PepMute_GFP_siRNA_U2OS

Figure4.PepMute™TransfectionReagentknockeddownstableGFPexpressioninMCF7cell(upperpanel)andU2OScell(lowerpanel)byreverselytransfecting5.0and1.0nMGFPsiRNArespectively.Greenfluorescenceprotein(GFP)wasstablyexpressedinMCF7andU2OScells.siRNAtargetingGFPgene(rightpanel)andashamsiRNA(leftpanel)wereintroducedintoMCF7andU2OScellswithfinal5.0and1.0nMrespectivelybyreversetransfectionwithPepMute™TransfectionReagent.GFPgenesilencingwasmonitored48hposttransfectionbyaNikonfluorescencemicroscope.QuantitativeanalysisshowedthatGFPsiRNAat5.0and1.0nMdeliveredbyPepMute™siRNATransfectionReagentknockeddown90%and95%stablyexpressedGFPinMCF7andU2OScellsrespectively.

DataSheet&Protocol
-AStandardsiRNATransfectionProtocol
-AStandardsiRNA/DNACo-transfectionProtocol
-AReversesiRNATransfectionProtocolforHTS

Torequestafreetrialsample,pleaseCreateAnAccountwithustoenteryourshippingaddressandemailusatorder@Signagen.com

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Testimonials:

IhadtriedyourproductpepMutetransfectionreagentonprimaryretinalneuronsandwassatisfiedwiththetransfectionefficiency.Willorder!

-VijaiKrishnanPh.D,LouisianaStateUniversity


IreallyappreciateyousendingmeasampleofPepMutesiRNAreagent.ItestedDNA/siRNAco-transfectionusing293Tcellsandtheresultswerecompletelysatisfactory.Iwasabletoget95%knockdownofmytargetgeneat1.0nMsiRNAaswellasexpressionofplasmidDNAusingtherecommendedprotocol.Iwillhavetotestandseeifothercelllinesarealsoaseffective.IwilldefinitelythinkaboutmovingtousethisreagentoverDharmafectorOligofectamine.

-HARISHN.RAMANATHANPh.D.,NIDDK,NIH


ItriedPepMutereagentandIlikeit.Ithassohighefficiencyandnocytotoxicity.Iamgoingtouseit.Thankforintroducingittoourlab.

-RadmilaHrdlickova,Ph.D.,UTAustin


Tried3differentsiRNAswith100%silencingonA2780cell.Thatisamazing!Ialreadyplacedanorder.

-DorisBenbrook,Ph.D.,OUHSC

 

Yes,IcertainlydidusethatsampleofPepMuteyougenerouslysentus…anditworkedsowellonHepG2cellsthatIhavesinceorderedafullsizeandamusingitexclusivelyforsiRNA! IcomparedtoRNAiMAXandRoche’sX-tremeGENEproducts,buttheseproductswerenotaseffectiveasPepMute. Thanksagainforthesample. Itwasfantastic!

-MatthewJackson, Ph.D.,USDA

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 07230861135606)SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。

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