Signagen/PolyJet In Vitro DNA Transfection Reagent/SL100688/0.1 mL,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/PolyJet In Vitro DNA Transfection Reagent/SL100688/0.1 mL

产品编号：SL100688-5x1.0mL

市场价：¥13100.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$655.00

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

PolyJetDNAInVitroTransfectionReagent

Description
Basedonourproprietarypolymersynthesistechnology,PolyJetDNAInVitroTransfectionReagentisformulatedasabiodegradablepolymerbasedDNAtransfectionreagentthatensureseffectiveandreproducIBLetransfectiononHEK293,COS-7,NIH-3T3,HeLa,CHOandabroadrangesofhard-to-transfectmammaliancells.PolyJetreagentisabletoimmobilizeDNAmigrationduringelectrophoresisatverylowconcentrationandformtransfectioncomplexwithin5minutesatRT.Aremarkablefeatureofthereagentistherapidandcompletedegradationofpolymeraftertransfectioncomplexendocytosis(Figure1),leADIngtomuchlesscytotoxicity.PolyJetreagent,1.0ml,issufficientfor~667transfectionsin24wellplatesor~333transfectionsin6wellplates,providingaveryaffordablealternativetotheleadingproductsfortransfectingavarietyofcommonlyusedandhard-to-transfectmammaliancells.

Figure1.ACartoonShowingBiodegradationofPolyJetDNATransfectionReagentAfterEndocytosisofTransfectionComplex

Features
-Bio-degradableafterendocytosis
-Exceptionalhightitersofvirusproduction
-EquallygoodforverylongDNAs(>89kb)
-EquallygoodforbothsingleDNAtransfectionandmultiDNAco-transfection
-Highlevelsofrecombinantproteinproduction
-Simple&robusttransfectionprocedure
-Veryaffordable

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.

BroadTransfectionSpectrumforMammalianCellTypes

CellLines	Efficiency(%GFP)	CellLines	Efficiency(%GFP)
McArdle7777 Hep3D SHEP 3T3-442A COS-7 CV-1 D407 DHDPro.b 3LL B16-F10 BAEC BHK-21 CaSki CaCo2 CHO HCS-2/8 HEK-293 HeLa HLMEC H-MVEC Huh-7D12 ATT20 SK-N-SH C2C12 HepG2	65-70% 67-76% 68-71% 35% 85-90% 60% 70% 70% 80% 85% 51% 80% 88% 60% 88% 61% 86% 88% 72% 59% 72% 46% 29% 46% 72%	hESCs SN56 MC3T3-E1 Primarymelanocyte mESCs L929 MCF-7 MDCK Neuro2A NIH3T3 PC12 SH-SY5Y SiHa SKOV3 Huh-7 IGROV1 DF-1,ChickenEmbryonicCell 6CSFMEo WEHI231 A549 LNCap Prim.mousekeratinocyte Prim.humanskinfibroblast Prim.humanpre-adipocyte Prim.mouseembry.fibroblast	70% 81% 80% 35% 70% 59% 68% 68% 86% 76% 50% 25% 60% 65% 70% 35% 50% 71% 26% 75% 75 29% 50% 32% 30%

ExamplesShowingTransfectionEfficiencyofPolyJetDNAInVitroTransfectionReagentonCommonlyUsedCells
PolyJet_L2K_CHO
TransfectionefficiencycomparisonofPolyJetvs.lipofectaminePlusonChineseHamsterOvary(CHO)cells.HAtaggedbeta-tubulinCDNAwasdeliveredintoCHOcellswithPolyJet(leftpanel)andlipofectaminePlus(rightpanel)respectively.FITCconjugatedantibodyagainstHAtagwasutilizedtopickupHA-beta-tubulin(Green)whileaDM1aantibodywasusedtodetectendogenousalpha-tubulinfollowedbyprobingwithrhodamineconjugatedsecondaryantibody(Red).TheabovepicturewasprovidedbyDr.ShangYinofUniversityofTexasatHoustonMedicalSchoolascourtesy

PolyJet_L2K_HEK293
AcomparisonshowingtransfectionefficiencyofPolyJetreagentvs.aleadingproduct,Lipofectamine2000onHEK293FTcells.HEK-293FTcellsweretransfectedwithGFPvector(pEGFP-N3)byPolyJet(leftpanel)andLipofectamine2000(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection

PolyJet_L2K_HepG2
AcomparisonshowingtransfectionefficiencyofPolyJetreagentvs.aleadingproduct,Lipofectamine2000onHepG2cells.HepG2cellsweretransfectedwithGFPvector(pEGFP-N3)byPolyJet(leftpanel)andLipofectamine2000(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection

PolyJet_Fugene_HD_MDCK
TransfectionefficiencycomparisonofPolyJetvs.FugeneHDonMDCKcells.AplainGFPDNAwastransducedintoMDCKcellswithPolyJet(leftpanel)andFugeneHD(rightpanel)reagentsrespectivelypermanufacturers"protocols.GFPandDAPIstainingwerevisualizedunderfluorescencemicroscopy48hoursposttansfection.TheabovecomparisondataandpictureswerecompletedandprovidedbyDr.GeZhouofNYUMedicalCenterascourtesy

PolyJet_L2K_MDCK
AcomparisonshowingtransfectionefficiencyofPolyJetreagentvs.aleadingproduct,Lipofectamine2000onMDCKcells.MDCKcellsarenotoriouslyhardtotransfect.Withproprietary"ShavedCellTransfection"protocol,PolyJet(leftpanel)givesupto70%GFPpositivecellsvs.Lipofectamine2000(rightpanel)around5%efficiency.MDCKcellsweretransfectedwithGFPvector(pEGFP-N3)byPolyJet(leftpanel)andLipofectamine2000(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope36hoursposttransfection

PolyJet_Fugene_HD_LNCap
AcomparisonshowingtransfectionefficiencyofPolyJetreagentvs.aleadingproduct,FugeneHDonLNCapcells.LNCapcellsweretransfectedwithGFPvector(pEGFP-N3)byPolyJet(leftpanel)andFugeneHD(rightpanel)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection

PolyJet_hESCs
AimageshowingexceptionaltransfectionefficiencyofPolyJetreagentonHumanembryonicstemcells (hESCs). ThehESCsgrowninE8mediumonGeltrexvs(leftpanel,DICimaging)wastransfectedwithpEF1α-GFP.TheGFPexpression(rightpanel)wasvisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection. TheabovepictureswereprovidedbyDr.MarinaPryzhkovaofJohnsHopkinsUniversityascourtesy

PolyJet_L2K_N2A
Neuro2AcellstransfectedwithpEGFP-C1plasmidusingPolyJetInVitroDNATransfectionReagent.TheNeuro2AcellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging(left)andFITCimaging(right)24hourspost-transfection

PolyJet_L2K_Primary_Fibroblast
ComparisonofcytotoxicityofPolyJetDNAInVitroTransfectionReagentwithL2Konprimarymurineskinfibroblast.Theprimarymurinefibroblastwasincubatedwiththeindicatedtransfectionreagents/pEGFP-C1(DNA)complexesabovefor4hoursinserum-freeDMEMHighGlucosemediumfollowedbyreplacementofcompleteserum-containingmedium.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscopewithDICphaseimaging24hoursposttransfection

DataSheetandProtocols
-GeneralProtocolforTransfectingMammalianCells
-AShortProtocolforTransfectingMammalianCells
-AdvancedProtocolforTransfectingHard-to-TransfectMammalianCells
-AProtocolforTransfectingSUSPension293andCHOCells
-ProtocolforLentivirusProduction
-ProtocolforrAAVProduction
- TechnicalNote&TransfectionTips

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Testimonials:

Sorryforsooobigdelay,butnowIamtotallyinlovewithPolyJet.IhaveusedPolyJetforawhileonmouseESCswithrelativelygoodefficiency50~70%.

--------MarinaPryzhkova,Ph.D.,JHU

PolyJetTransfectionReagentworkedequallyaswellaslipofectamine2000,withlittleevidenceofcelldeathon293,PC-3and22RV1cells.Iwilldefiantlyconsiderswitchingover.

-----TiffanyWallace,Ph.D.,NCI/NIH

Ionlydidsidebysidewiththetestingsample(PolyJet)andLipo2000withGFPtransfectiononCOS-7cells.Theresultwasverygood.PolyJetwasevenbetterthanL2K.

------FengQiao,Ph.D.,NEI/NIH

ItestedthesampleofPolyJetonmyNIH-3T3mousefibroblaststhisweekend.TheresultsweremuchbetterthanLipofecatmineLTX.I"mattachingapowerpointslidewithmyresults(Ididnotquantifythe%transfectionefficiency,butthepicturesgetthepointacross).Ifoundthattheprotocolfordifficult-to-transfectcelllinesworkedbetterthanthestandardprotocol.

-----StephanieMurphy,Ph.D.,DartmouthCollege

Ihadchancetotryyourproductfinally.Itwasgreatsuccess.IusedHeLacellsandgot10%transfectionefficiency(<0.1%forLipofectamine).Thankyou!IwaswonderingifIalsotryGenJetPlusDNAInVitroTransfectionReagent?Accordingtoyourwebsite,thereagentworksbetterthanregularPolyJet.

-------YumiUetake,Ph.D.,UMASS

ItestedPolyJetanditlooksgreatonMDCK.Weplacedorder.Thankyou!

-------GeZhou,Ph.D.,NYU

Wearehappytoprovidefeedback.PolyJetworkedverywellforusinHepG2cells,wegotapproximately80%efficiencywithpMAXGFPplasmid,byfollowingtheconditionsinyoursuggestedprotocol.WeranacomparisonwithLipofectamine,whichonlyshowedapproximately20-30%transfectionefficiency.Weareplanningexperimentsandwillbeorderingmoresoon.

-------EmilyMcallister,PBRC

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。