Signagen/rAAV Production Full Service, Medium Scale/SL100861/,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/rAAV Production Full Service, Medium Scale/SL100861/

产品编号：SL100861

市场价：¥0.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

rAAVProductionFullService,MediumScale

Features:
-Nonpathogenicwithleastimmuneresponse.
-Excellentgenedeliveryefficiencyinmostcelltypesincludingdividingandnon-dividingorprimarycells.
-Persistenttransgeneexpression.
-MultipleSEROtypes(AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-8,AAV-9,AAV-DJ/8andAAV-DJ).

Large AAVcisvectorinventory forconstructingAAVcisplasmidwith aspecificpromoterandreporter:

SinglepromoterrAAVcisvector		DualpromoterrAAVcisvector
Promoterless CMV CAG H1 U6 Synapsin UBC EF1α CBh TRE-miniCMV CMVtight ALB(1.4) MHCK7 ApoE/AAT1 CaMKII ELA1 Enh358MCK cTNT GFAP MBP SST TBG αMHC hRPE mIP1 tMCK		Promoterless CMV CAG H1 U6 Synapsin UBC EF1α CBh TRE-miniCMV CMVtight ALB(1.4) MHCK7 ApoE/AAT1 CaMKII ELA1 Enh358MCK cTNT GFAP MBP SST TBG αMHC hRPE mIP1 tMCK	CMV CAG H1 U6 Synapsin UBC EF1α CaMKII cTNT GFAP TRE-miniCMV CMVtight MHCK7	eGFP RFP mRFP mCherry tdTOMATO TurboGFP eYFP Venus Luc LacZ TRE-miniCMV CMVtight MHCK7

ServiceDescription:
-Cloningyourgeneofinterest(GOI)intoAAVcisvector.
-rAAVcisplasmidlargeprep.
-rAAVpackagingin1xcellstack.
-rAAVpurificationviaadvanced2xCsClultra-centrifugation.
-Desalting,filtersterilizationandAAVtitrationviaqPCR.

rAAVSerotypesWeOffer:
AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-7,AAV-8,AAV-9,AAV-PHP.B,AAV-DJ/8andAAV-DJ.

RequiredMaterials:ANCBIaccession#orgenesymbolorplasmidDNAcarryingyourgeneofinterest(GOI).

TurnaroundTime:2~3weeks.

Deliverables:>0.2mL(4x50µL)ofsuperpurifiedinvivograderAAVvectorat>1E+13VG/mL*.

Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.

SuperHighYield:
WithourproprietarygeneticallyengineeredAAV·HT™packagingcellandaadvancedpurificationapproach,therAAVyieldiseasytoreachsuperhighlevel--------total1E+15VG.ForsuperhighlevelrAAVproductionupto1E+15VGtotal,pleasecontactustorequestaquote.

Figure1.AcomparisonofpurityandinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedandsuperinfectious(closetoclinicaltrialgrade)rAAVvectorpreparedviaouradvanceddoubleCsClultra-centrifugationapproach.
A.rAAVvectors(total1E+9VGperlane) fromdifferentsourceswereresolvedonSDS-PAGEfollowedbysilverstaining. Lane1:GMPmanufacturedrAAVvectorfromCHOP;Lane2:rAAVpreparedviaouradvanced2xCsClultra-centrifugationapproach;Lane3:rAAVfromVectorCoreofBCM;Lane4:rAAVfromourcompetitor"V";Lane5:rAAVfromourcompetitor"C";Lane6:ProteinMarker.
B.SuperinfectiousrAAVvectorpreparedviaadvanceddoubleCsClultra-centrifugation. Leftpanel:rAAV9-GFP(total5E+9VG)fromourcompetitor"V"injectedtomouseeye;Rightpanel:rAAV-9-GFP(total5E+9VG)purifiedviaadvanced2xCsClultra-centrifugationinjectedtomouseeye.

Figure2.AcomparisonofinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedrAAVpreparedviaadvanceddoubleCsClultra-centrifugationapproachissuperinfectious.
rAAV1-GFP(total2E+9VG)fromdifferentsourceswereinjectedtomousemuscletissue. TheGFPfluorescencewasvisualized3weekspostinjection. A.rAAV1-GFPfromourcompetitor"V";B.rAAV1-GFPfromourpre-maderAAVsstockpurifiedviaadvanceddoubleCsClultra-centrifugation. C.QuantificationdatashowedthatoursuperpurifiedrAAV(bar2)is~9timesmoreinfectiousthanthat(bar1)preparedviaconventionalCsClultra-centrifugation.

*Finalviralyieldmaydependonthenatureoftransgene.

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。