shRNAValidationServiceviaCell-basedReporterSystem:
-CloningORFofgeneofinterests(GOI)intoanadenovirusshuttlevectorwithareporter.
-Designandsynthesize~6shRNAoligoesfollowedbycloningthemtotheaboveshuttleconstructtoobtainshRNAvalidationplasmid.
-TransienttransfectiontheshRNAvalidationplasmid.
-QuantificationoftargetgenesilencingviaFACS(forGFPexpression)orluminometer(forluciferaseexpression).
AvailableshRNAValidationPlatforms:
-ConstitutiveshRNAknockdown
-InducIBLeshRNAknockdown
GuaranteedKnockdown:
Weguaranteeatleast1shRNAat>80%knockdownofmRNAlevelinthetestedcellsbyscreening~6shRNAoligoes.
Weofferdiscountfornewcustomer,please requestaquotewithustoday.
Figure1. ACartoonShowingshRNAValidationProcessforshRNAAdenovirusProduction.
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606),SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。