商品介绍
-Excellentgenedeliveryefficiencyinmostcelltypesincludingdividingandnon-dividingorprimarycells.
-MultipleSEROtypes(AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-8,AAV-9,AAV-DJ/8andAAV-DJ).
-Persistenttransgeneexpression.
-Induceefficientgenesilencing.
ServiceDescription:
-Largescaleco-transfectionofAAV·HT293cellsinto2xcellstack.
-HarvestrAAVfollowedbypurificationvia2xCsClultra-centrifugation.
-TitrationviaqPCR(measuringgenomecopies).
rAAVSerotypesWeOffer:
AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-7,AAV-8,AAV-9,AAV-PHP.B,AAV-DJ/8andAAV-DJ.
RequiredMaterials:
rAAVcisplasmidcarryingyourshRNAorpre-miRNA,>300µg.
TurnaroundTime:
1~2week.
Deliverables:>1.0mLsuperpurifiedinvivograderAAVvectorat>1E+13VG/mL*.
Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.
WealsooffertruncatedcustomshRNAAAVservice,pleaserequestaquotewithustoday.
Figure1.AcomparisonofpurityandinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedandsuperinfectious(closetoclinicaltrialgrade)rAAVvectorpreparedviaouradvanceddoubleCsClultra-centrifugationapproach.
A.rAAVvectors(total1E+9VGperlane) fromdifferentsourceswereresolvedonSDS-PAGEfollowedbysilverstaining. Lane1:GMPmanufacturedrAAVvectorfromCHOP;Lane2:rAAVpreparedviaouradvanced2xCsClultra-centrifugationapproach;Lane3:rAAVfromVectorCoreofBCM;Lane4:rAAVfromourcompetitor"V";Lane5:rAAVfromourcompetitor"C";Lane6:ProteinMarker.
B.SuperinfectiousrAAVvectorpreparedviaadvanceddoubleCsClultra-centrifugation. Leftpanel:rAAV9-GFP(total5E+9VG)fromourcompetitor"V"injectedtomouseeye;Rightpanel:rAAV-9-GFP(total5E+9VG)purifiedviaadvanced2xCsClultra-centrifugationinjectedtomouseeye.
Figure2.AcomparisonofinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedrAAVpreparedviaadvanceddoubleCsClultra-centrifugationapproachissuperinfectious.
rAAV1-GFP(total2E+9VG)fromdifferentsourceswereinjectedtomousemuscletissue. TheGFPfluorescencewasvisualized3weekspostinjection. A.rAAV1-GFPfromourcompetitor"V";B.rAAV1-GFPfromourpre-maderAAVsstockpurifiedviaadvanceddoubleCsClultra-centrifugation. C.QuantificationdatashowedthatoursuperpurifiedrAAV(bar2)is~9timesmoreinfectiousthanthat(bar1)preparedviaconventionalCsClultra-centrifugation.
*Finalviralyieldmaydependonthenatureoftransgene.
品牌介绍
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。
公司介绍
产品分类
Protein Kianses
Cytochrome P450
Lentivirus Production Service
Endopeptidases & Exopeptidases
Protein Kinase, Dead Mutants or Dominant Negatives
Cytokines
Western Blotting Products
Pre-made Lentivirus
Adenovirus Production Service
Phosphoric Diester Hydrolases
Clones for Signal Transduction
Phosphoprotein Phosphatases
Pre-made AAVs
In Vitro DNA Transfection Reagents
Ion Channels
Pre-optimized Transfection Reagents
In Vitro siRNA Transfection Reagents
Tumor and Apoptosis
In Vivo Transfection Reagents
Membrane Receptors
shRNA/miRNA Adenovirus Svc
Ligand-dependent Nuclear Receptors
Pre-made Adenovirus
AAV Production Service
Transfection Accessories
Free Offers
shRNA/miRNA AAV Service
Miscellaneous Ready to Package Adenoviruses
Transcription Factors
G-protein Coupled Receptors
销售排行
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全球直采 正品优价
正品保障
厂家直发 有线跟踪
正规清关
CIF100%正规报关，提供发票
及时交付
限时必达 不达必赔
总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
工作时间：周一至周五9:00~18:00
技术支持：
短信：18915418616
服务热线：
4000-520-616（全国）
☏ 0512-67156496（苏州）
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