shRNA/miRNAValidationServiceviaCell-basedReporterSystem:
-CloningORFofgeneofinterests(GOI)intoarAAVcisvectorwithareporter.
-Designandsynthesize~6shRNAoligoesfollowedbycloningthemtotheaboverAAVcisconstructtoobtainshRNAvalidationplasmids.
-FormiRNAvalidation,synthesizepre-miRNAoligoesfollowedbycloningthemtotheaboverAAVcisconstructtoobtainshRNAvalidationplasmid.
-TransienttransfectiontheshRNA/miRNAvalidationplasmids.
-QuantificationoftargetgenesilencingviaFACS(forGFPexpression)orluminometer(forluciferaseexpression).
AvailableshRNA/miRNAValidationPlatforms:
-ConstitutiveshRNAknockdown
-InducIBLeshRNAknockdown
GuaranteedKnockdown:
Weguaranteeatleast1(one)shRNAwith>80%knockdownofmRNAlevelinthetestedcellsbyscreening~6shRNA/miRNAoligoes.
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Figure1. ACartoonShowingshRNAValidationProcessforshRNArAAVProduction.
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606),SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。