ProductionofRecombinantSEROtype5shRNA/miRNAAdenovirus(E1/E3deletion)withAd.MAXSystem:
-One-stopservice:fromshRNA/miRNAdesigntopackaging.
-Unsurpassabletransductionefficiency.
-Quickturnaroundtime:3to4weeks.
-Cost-effective.
-Specialdesignrequestacceptable,FREE!
LargeshRNA/miRNA/gRNAAd.MAX™shuttlevectorcollectionforcloningshuttleplasmid with aspecificpromoterandreporter:
Single-promoterAd.MAX™shuttlevector | Dual-promoterAd.MAX™shuttlevector | |||
Promoterless |
| Promoterless | CMV | eGFP |
ServiceDescription:
1.SynthesizeandcloneshRNA/miRNAtoashuttlevectorfollowedbytransferofthewholetransgenecassettetoadenoviralgenome.
2.ViralproductioninAd.MAX™293cells:transfectionandpurificationofrecombinantadenovirus.
3.Generationofhightiterrecombinantadenoviralstockin~500cm2Ad.MAX™293cells.
4.RecombinantadenovirusPFUtitration.
RequiredMaterials:NCBIaccession#oftargetgene,targetregionsequence,shRNAorsiRNAsequencevalidated/publishedormaturemiRNAsequence,miRNAaccession#orgRNAsequenceetc.
TurnaroundTime:3~4weeks.
Deliverables:1.0mL(5x0.2mL)ofsuperinfectiousinvitrogradeadenovirusvectorat5E+10~5E+11PFU/mL*.
Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.
WealsooffertruncatedcustomshRNA/miRNA/gRNAadenovirusservice,pleaserequestaquotewithustoday.
*Finalviralyieldmaydependonthetransgeneinthevirus.
SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606),SignaGen已经成功开发出三大类 DNA/siRNA转染试剂,经验证我们的产品比市场上主导产品效率更高,细胞毒性更低。更重要的是我们提供一个合理的价位。