Signagen/shRNA/miRNA/gRNA rAAV Production Full Service, Pilot Scale/SL100874/,Signagen,,Signagen,SignaGen Laboratories,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Signagen/shRNA/miRNA/gRNA rAAV Production Full Service, Pilot Scale/SL100874/

产品编号：SL100874

市场价：¥0.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞研究>细胞转染>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Signagen

公司：SignaGen Laboratories

公司分类：

商品介绍

shRNA/miRNA/gRNArAAVProductionFullService,PilotScale

Features:
-Excellentgenedeliveryefficiencyinmostcelltypesincludingdividingandnon-dividingorprimarycells.
-MultipleSEROtypes(AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-8,AAV-9,AAV-DJ/8andAAV-DJ).
-Persistenttransgeneexpression.
-Induceefficientgenesilencing.

LargeshRNA/miRNA/gRNAAAVcisvectorinventoryforconstructingAAVcisplasmidwithaspecificpromoterandreporter:

ForConventionalshRNA/miRNA/TuDrAAVProduction
Single-promotershRNA/miRNAAAVcisvector			Dual-promotershRNA/miRNArAAVcisvector
Promoterless CMV CAG H1 U6 Synapsin UBC EF1α ALB(1.4) ApoE/AAT1 CaMKII ELA1 Enh358MCK cTNT GFAP MBP SST TBG αMHC hRPE mIP1 tMCK			Promoterless CMV CAG H1 U6 Synapsin UBC EF1α ALB(1.4) ApoE/AAT1 CaMKII ELA1 Enh358MCK cTNT GFAP MBP SST TBG αMHC hRPE mIP1 tMCK	CMV CAG H1 U6 Synapsin UBC EF1α CaMKII cTNT GFAP	eGFP RFP mRFP mCherry tdTOMATO TurboGFP eYFP Venus Luc LacZ
ForCRISPR/Cas9rAAVProduction
Single-promoterCRISPR/Cas9AAVcisvector			Dual-promoterCRISPR/Cas9AAVcisvector
Promoterless U6 H1 CMV CAG CBH Synapsin UBC EF1α ALB(1.4) ApoE/AAT1 CaMKII ELA1 Enh358MCK cTNT GFAP MBP SST TBG αMHC hRPE mIP1 tMCK	Empty CBH U6 H1 sCMV Syn sEF1α CK0.4 cTNT GFAP0.6 sSyn SST TBG αMHC hRPE mIP1 tMCK CBH	Cas9 hCas9 SpCas9 hSpCas9 SaCas9 AsCpf1 hAsCpf1 ftCas9 st1Cas9 cjCas9 cdCas9 ciCas9 ncCas9 st3Cas9 nmCas9 mgCas9 nsCas9	Promoterless sCMV U6 H1 tRNA Syn sSyn sEF1α CaMKII0.4	CMV sCMV CBH H1 U6 Syn sSyn sEF1α CaMKII0.4	Cas9 hCas9 SpCas9 hSpCas9 SaCas9 AsCpf1 hAsCpf1 ftCas9 st1Cas9 cjCas9 cdCas9 ciCas9 ncCas9 st3Cas9 nmCas9 mgCas9 nsCas9

ServiceDescription:
1.SynthesizeandcloneshRNAintoAAVcisvectors.
2.PilotscaletransfectionofAAV·HT™293cellinto10x150mmplates.
3.HarvestrAAVfollowedbypurificationviaadvanced2xCsClultra-centrifugationtoobtainclinicaltrialgradeofrAAVvector.
4.Desalting,filtersterilization,andAAVtitrationviaqPCR.

rAAVSerotypesWeOffer:
AAV-1,AAV-2,AAV-3,AAV-4,AAV-5,AAV-6,AAV-7,AAV-8,AAV-9,AAV-PHP.B,AAV-DJ/8andAAV-DJ.

RequiredMaterials:NCBIaccession#,targetsequence,validatedsiRNAorvalidatedshRNAsequence;miRNAaccession#,pre-miRNA,maturemiRNAorTuD(toughdecoy)miRNA.

TurnaroundTime:3~4weeks.

Deliverables:>0.1mLsuperpurifiedinvivogradeofrAAVvectorat>1E+13VG/mL*.

Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.

WealsooffertruncatedcustomshRNA/miRNAAAVservice,pleaserequestaquotewithustoday.

Figure1.AcomparisonofpurityandinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedandsuperinfectious(closetoclinicaltrialgrade)rAAVvectorpreparedviaouradvanceddoubleCsClultra-centrifugationapproach.
A.rAAVvectors(total1E+9VGperlane) fromdifferentsourceswereresolvedonSDS-PAGEfollowedbysilverstaining. Lane1:GMPmanufacturedrAAVvectorfromCHOP;Lane2:rAAVpreparedviaouradvanced2xCsClultra-centrifugationapproach;Lane3:rAAVfromVectorCoreofBCM;Lane4:rAAVfromourcompetitor"V";Lane5:rAAVfromourcompetitor"C";Lane6:ProteinMarker.
B.SuperinfectiousrAAVvectorpreparedviaadvanceddoubleCsClultra-centrifugation. Leftpanel:rAAV9-GFP(total5E+9VG)fromourcompetitor"V"injectedtomouseeye;Rightpanel:rAAV-9-GFP(total5E+9VG)purifiedviaadvanced2xCsClultra-centrifugationinjectedtomouseeye.

Figure2.AcomparisonofinfectivityofrAAVvectorsfromdifferentsourcesshowingsuperpurifiedrAAVpreparedviaadvanceddoubleCsClultra-centrifugationapproachissuperinfectious.
rAAV1-GFP(total2E+9VG)fromdifferentsourceswereinjectedtomousemuscletissue. TheGFPfluorescencewasvisualized3weekspostinjection. A.rAAV1-GFPfromourcompetitor"V";B.rAAV1-GFPfromourpre-maderAAVsstockpurifiedviaadvanceddoubleCsClultra-centrifugation. C.QuantificationdatashowedthatoursuperpurifiedrAAV(bar2)is~9timesmoreinfectiousthanthat(bar1)preparedviaconventionalCsClultra-centrifugation.

*Finalviralyieldmaydependonthenatureoftransgene.

品牌介绍

SignaGen公司是一家专注于开发和生产基因转导工具的企业,服务于生物医学研究领域。借助我们独有的技术平台(美国专利商标局号码为 072308和61135606)，SignaGen已经成功开发出三大类 DNA/siRNA转染试剂，经验证我们的产品比市场上主导产品效率更高，细胞毒性更低。更重要的是我们提供一个合理的价位。