货号： SL100489-SiHa
保存条件： 4°C
供应商： 济南美中清水湾生物科技有限公司
数量： 现货 大量
英文名： GenJet™ In Vitro DNA Tranfection Reagent for SiHa Cells
保质期： 一年
规格： 毫升/支
DescriptionGenJet™ DNA In Vitro Tranfection Reagent for SiHa is pre-optimized for transfecting SiHa cells.  Refer to the following optimal transfection conditions for maximal transfection efficiency on SiHa cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:Confluence on the day of transfectionCell culture conditionsGenJet™ (µl) : DNA (µg) RatioDiluent for DNA and Transfection ReagentIncubation Time to Form GenJet™/DNA ComplexPresence of Serum/Antibiotics during TransfectionChange Medium After TransfectionMaximal Efficiency Transfection Results:Reporter GenePlasmidEfficiency (GFP %)~80%DMEM with 4.5 g/L glucose, 10% FBS3:1Serum-free DMEM with 4.5 g/L glucose15 minutes at RTYesYes, ~5 Hours After Transfection48 hoursEGFPpEGFP-N3 (CMV promoter) 76%
Storage ConditionStore at 4 °C. If stored properly, the product is stable for 12 months or longerData Sheet   温馨提示：不可用于临床治疗。