您知悉并同意，丁香通平台展示产品并非为平台自身产品，您发起询价或试用申请后，平台将会把您已提供或按要求提供的相关信息同步提供至所咨询的具体产品商家，由商家为您提供具体产品的价格咨询或产品试用注意事项及相关试用产品或完成相应购买，因此您知悉并授权平台将您的信息进行同步共享。您有权拒绝，但您知悉将无法参加询价或试用活动。 DescriptionGenJet™ DNA In Vitro Tranfection Reagent for HepG2 Cells is pre-optimized for HepG2 cell transfection. HepG2 (Hepatocellular carcinoma, human) is a perpetual add cell line which was derived from the liver tissue of a 15 year old caucasian male with a well differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a model chromosome number of 55 and are not tumorigenic in nude mice. The cells secrete a variety of major plasma proteins e.g.. albumin, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. HepG2 cells have been shown to be G418 resistant (400µg/mL). The cells will respond to stimulation with human growth hormone. Refer to the following optimal transfection conditions for maximal transfection efficiency on HepG2 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates. Summary of Optimal Transfection Conditions: HepG2 cell culture Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium 5 Hours After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %)collagen type I pre-treated culture dish ~90% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes No 48 hours EGFP pEGFP-N3 (CMV promoter)  82% Storage ConditionStore at 4 °C. If stored properly, the product is stable for 12 months or longerA Picture Showing Transfection Efficiency of GenJet™ Reagent on HepG2 CellsGenJet™ reagent is optimized for HepG2 cells (ATCC # HB-8065) with exceptional efficiency in comparison of Lipofectamine 2000 (L2K) and Amaxa electroporation device. HepG2 cells were grown per ATCC recommended culture medium on a collagen type I treated culture dish and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 48 hours post transfection. Right Panel: Comparison of transfection efficiency of GenJet with Lipofecatmine 2000 (L2K), and Amaxa electroporation device on HepG2 cells. GFP DNA (pEGFP-N3, 4.7 kb) was introduced to HepG2 cell (cultured on Collagen pretreated dishes) with different transfection reagents per manufacturer\'s protocols . GFP positive cell (%) and fluorescence intensity were detected by passing through FACS 48 hours post transfection Left Panel: presence of serum and antibiotics enhances GenJet reagent\'s efficiency on HepG2 cells. HepG2 cell (grown on collagen treated dishes) was transfected with three different conditions-------serum and antibiotics free, presence of 10% serum and antibiotics followed by removal 5 hours post transfection and presence of 10% serum and antibiotics without removal 5 hours post transfection. GenJet™ reagent is optimized for HepG2 cells (ATCC # HB-8065). HepG2 cells were grown per ATCC recommended culture medium on a collagen type I treated culture dish and transfected with GFP (pEGFP-N3, 4.7 kb, right panel) and β-galactosidase cDNA (pSV-β-galactosidase, 6.9 kb, left panel) by pre-optimized GenJet™ DNA Transfection Reagent for HepG2 cells . The efficiency was checked 48 hours post transfection by Zeiss 510 Confocal Microscopy and β-galactosidase staining kit respectively. Data Sheet TestimonialsIt has been tested and better results were obtained than with Amaxa Nucleofactor. A colleague and I, will be using it for all of our transfection experiments. ----------Fouad Francis., Ph.D., NICHD/NIHI have got 70% efficiency with a GFP plasmid. With my protein of interest, which is 200KDa, I got 20% efficiency, pretty comparable to Amaxa electroporation. Thank you SignaGen. --------Marina Uhart, Ph.D., NHLBI/NIHWow!!!!! What a product. We have been using Lipofectamine 2000 on HepG2 and have been struggling to get even modest transfection efficiency. In contrast, GenJet gave us dramatically higher transfection efficiency. By eye, looks like 5-10 fold improvement. The especially impressive part is that one uses less reagent and less DNA to get much better results. Our future HepG2 experiments will now all be done with GenJet. Thanks.--------Erik Snapp, Ph.D., Albert Einstein College of Medicine.  温馨提示：不可用于临床治疗。 风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。